DNA vaccines with single-chain Fv fused to fragment C of tetanus toxin induce protective immunity against lymphoma and myeloma

被引：222

作者：

King, CA ^{[1
]}

Spellerberg, MB ^{[1
]}

Zhu, DL ^{[1
]}

Rice, J ^{[1
]}

Sahota, SS ^{[1
]}

Thompsett, AR ^{[1
]}

Hamblin, TJ ^{[1
]}

Radl, J ^{[1
]}

Stevenson, FK ^{[1
]}

机构：

[1] Southampton Univ Hosp Trust, Tenovus Lab, Mol Immunol Grp, Southampton SO16 6YD, Hants, England

来源：

NATURE MEDICINE | 1998年 / 4卷 / 11期

关键词：

D O I：

10.1038/3266

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vaccination with idiotypic protein protects against B-cell lymphoma, mainly through anti-idiotypic antibody. For use in patients, DNA vaccines containing single-chain Fv derived from tumor provide a convenient alternative vaccine delivery system. However, single-chain Fv sequence alone induces low anti-idiotypic response and poor protection against lymphoma. Fusion of the gene encoding fragment C of tetanus toxin to single-chain Fv substantially promotes the anti-idiotypic response and induces strong protection against B-cell lymphoma. The same fusion design also induces protective immunity against a surface Ig-negative myeloma. These findings indicate that fusion to a pathogen sequence allows a tumor antigen to engage diverse immune mechanisms that suppress growth. This fusion design has the added advantage of overcoming potential tolerance to tumor that may exist in patients.

引用

页码：1281 / 1286

页数：6

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