Recognition and maturation of effector RNAs in a CRISPR interference pathway

被引：123

作者：

Gesner, Emily M. ^{[1
]}

Schellenberg, Matthew J. ^{[1
]}

Garside, Erin L. ^{[1
]}

George, Mark M. ^{[1
]}

MacMillan, Andrew M. ^{[1
]}

机构：

[1] Univ Alberta, Dept Biochem, Edmonton, AB, Canada

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2011年 / 18卷 / 06期

基金：

加拿大自然科学与工程研究理事会;

关键词：

ESCHERICHIA-COLI; SPLICING ENDONUCLEASE; MAXIMUM-LIKELIHOOD; STRUCTURAL BASIS; PROKARYOTES; PROTEIN; CLEAVAGE; SEQUENCE; DEFENSE; DNA;

D O I：

10.1038/nsmb.2042

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In bacteria and archaea, small RNAs derived from clustered, regularly interspaced, short palindromic repeat (CRISPR) loci are involved in an adaptable and heritable gene-silencing pathway. Resistance to phage infection is conferred by the incorporation of short invading DNA sequences into the genome as CRISPR spacer elements separated by short repeat sequences. Processing of long primary transcripts (pre-crRNAs) containing these repeats by an RNA endonuclease generates the mature effector RNAs that interfere with phage gene expression. Here we describe structural and functional analyses of the Thermus thermophilus CRISPR Cse3 endonuclease. High-resolution X-ray structures of Cse3 bound to repeat RNAs model both the pre- and post-cleavage complexes associated with processing the pre-crRNA. These structures establish the molecular basis of a specific CRISPR RNA recognition and suggest the mechanism for generation of effector RNAs responsible for gene silencing.

引用

页码：688 / U83

页数：6

共 31 条

[21] Refinement of macromolecular structures by the maximum-likelihood method [J].