The direct electrochemistry of cytochrome c at a hanging mercury drop electrode modified with 6-mercaptopurine

被引：31

作者：

Sevilla, JM ^{[1
]}

Pineda, T ^{[1
]}

Roman, AJ ^{[1
]}

Madueno, R ^{[1
]}

Blazquez, M ^{[1
]}

机构：

[1] Univ Cordoba, Fac Ciencias, Dept Quim Fis & Termodinam Aplicada, Cordoba 14004, Spain

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1998年 / 451卷 / 1-2期

关键词：

mercury electrode; 6-mercaptopurine; cytochrome c; electrochemistry;

D O I：

10.1016/S0022-0728(98)00075-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The modification of a mercury electrode with 6-mercaptopurine (6MP) has been performed at pH 6 in acetate buffer and the electrode behaviour has been checked in the absence of the modifier in solution. Direct electrochemistry of cytochrome c has been observed on a 6MP-Hg modified electrode. At high coverage of 6MP, the monolayer acts as an effective promoter for the redox exchange of cyt c, which is not observed on a bare mercury electrode. The film is stable for more than 200 scans and must play a role on avoiding irreversible adsorption and denaturation of the protein. At pH 7, 25 mM phosphate buffer and 0.1 M NaClO4, cyt c exhibits a quasi-reversible electron exchange similar to that reported with gold modified electrodes, the electron rate constant being 4.1 x 10(-3) cm s(-1). As far as we know it is the first time that such a response has been observed on mercury. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：89 / 93

页数：5