Suppressive effects of SP-A on ionomycin-induced IL-8 production and release by eosinophils

被引:23
作者
Cheng, G [1 ]
Ueda, T [1 ]
Nakajima, H [1 ]
Nakajima, A [1 ]
Kinjyo, S [1 ]
Motojima, S [1 ]
Fukuda, T [1 ]
机构
[1] Dokkyo Univ, Sch Med, Dept Internal Med & Clin Immunol, Mibu, Tochigi 32102, Japan
关键词
surfactant protein a; eosinophils; IL-8; ELISA;
D O I
10.1159/000053574
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Recent studies have demonstrated that pulmonary surfactant protein (SP)-A plays a potential role in modifying inflammation and immune function. To see whether SP-A could modify IL-8 production and release by eosinophils stimulated with ionomycin, SP-A purified from surfactant recovered from patients with alveolar proteinosis was added to eosinophils isolated by the negative-selection method with immunomagnetic beads, and cultured for 24 h. The concentrations of IL-8 in the cell-free supernatants and cell lysates were then measured by ELISA. SP-A attenuated the production of IL-8 by eosinophils in a concentration-dependent manner. SP-A also attenuated the release of IL-8 from the eosinophils. The addition of SP-A antibody (PE10) reversed these effects of SP-A completely. These data suggest that SP-A may have the potential to modify allergic inflammation by inhibiting the release and production of IL-8 by eosinophils.
引用
收藏
页码:59 / 62
页数:4
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