Structure of an avian influenza A virus NS1 protein effector domain

被引:81
作者
Hale, Benjamin G. [1 ]
Barclay, Wendy S. [3 ]
Randall, Richard E. [1 ,2 ]
Russell, Rupert J. [1 ,2 ]
机构
[1] Univ St Andrews, Ctr Biomol Sci, St Andrews KY16 9ST, Fife, Scotland
[2] Univ St Andrews, Interdisciplinary Ctr Human & Avian Influenza Res, St Andrews KY16 9ST, Fife, Scotland
[3] Univ London Imperial Coll Sci Technol & Med, Dept Virol, Fac Med, London W2 1PG, England
基金
英国医学研究理事会;
关键词
avian influenza; NS1; X-ray crystallography; dimerization; interferon-antagonist;
D O I
10.1016/j.virol.2008.05.026
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Influenza A virus NS1 protein is a multifunctional virulence factor. Here, we report a crystal structure for the NS1 effector domain of avian influenza virus A/Duck/Albany/76. Comparison of this structure with that reported for a human strain shows both proteins share a common monomer conformation, albeit with subtle differences. Strikingly, our data reveal a novel helix-helix dimeric interface between monomers of the avian NS1 protein, which is also found in the human NS1 crystal lattice. We re-evaluate the current model of NS1 dimeric assembly, and provide biochemical evidence to show tryptophan-187 (a residue located at the helix-helix interface) is essential for dimerization of this effector domain. (c) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:1 / 5
页数:5
相关论文
共 23 条
[1]   PHENIX:: building new software for automated crystallographic structure determination [J].
Adams, PD ;
Grosse-Kunstleve, RW ;
Hung, LW ;
Ioerger, TR ;
McCoy, AJ ;
Moriarty, NW ;
Read, RJ ;
Sacchettini, JC ;
Sauter, NK ;
Terwilliger, TC .
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2002, 58 :1948-1954
[2]   THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].
BAILEY, S .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763
[3]   X-ray structure of influenza virus NS1 effector domain [J].
Bornholdt, Zachary A. ;
Prasad, B. V. Venkataram .
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2006, 13 (06) :559-560
[4]   PABP1 and elF4Gl associate with influenza virus NS1 protein in viral mRNA translation initiation complexes [J].
Burgui, I ;
Aragón, T ;
Ortín, J ;
Nieto, A .
JOURNAL OF GENERAL VIROLOGY, 2003, 84 :3263-3274
[5]   A recombinant influenza A virus expressing an RNA-binding-defective NS1 protein induces high levels of beta interferon and is attenuated in mice [J].
Donelan, NR ;
Basler, CF ;
García-Sastre, A .
JOURNAL OF VIROLOGY, 2003, 77 (24) :13257-13266
[6]   Influenza A virus NS1 protein binds p85β and activates phosphatidylinositol-3-kinase signaling [J].
Hale, Benjamin G. ;
Jackson, David ;
Chen, Yun-Hsiang ;
Lamb, Robert A. ;
Randall, Richard E. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (38) :14194-14199
[7]   NS1 proteins of avian influenza A viruses can act as antagonists of the human alpha/beta interferon response [J].
Hayman, A. ;
Comely, S. ;
Lackenby, A. ;
Hartgroves, L. C. S. ;
Goodbourn, S. ;
McCauley, J. W. ;
Barclay, W. S. .
JOURNAL OF VIROLOGY, 2007, 81 (05) :2318-2327
[8]   Variation in the ability of human influenza A viruses to induce and inhibit the IFN-β pathway [J].
Hayman, A ;
Comely, S ;
Lackenby, A ;
Murphy, S ;
McCauley, J ;
Goodbourn, S ;
Barclay, W .
VIROLOGY, 2006, 347 (01) :52-64
[9]   Avian and 1918 Spanish influenza a virus NS1 proteins bind to Crk/CrkL src homology 3 domains to activate host cell signaling [J].
Heikkinen, Leena S. ;
Kazlauskas, Arunas ;
Melen, Krister ;
Wagner, Ralf ;
Ziegler, Thedi ;
Julkunen, Ilkka ;
Saksela, Kalle .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (09) :5719-5727
[10]   A new influenza virus virulence determinant: The NS1 protein four C-terminal residues modulate pathogenicity [J].
Jackson, David ;
Hossain, Md. Jaber ;
Hickman, Danielle ;
Perez, Daniel R. ;
Lamb, Robert A. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (11) :4381-4386