2-Hydrazino-1-methylpyridine: a highly sensitive derivatization reagent for oxosteroids in liquid chromatography-electrospray ionization-mass spectrometry

被引：79

作者：

Higashi, T ^{[1
]}

Yamauchi, A ^{[1
]}

Shimada, K ^{[1
]}

机构：

[1] Kanazawa Univ, Grad Sch Nat Sci & Technol, Div Pharmaceut Sci, Kanazawa, Ishikawa 9201192, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 825卷 / 02期

关键词：

derivatization; LC-ESI-MS; oxosteroids; human prostate;

D O I：

10.1016/j.jchromb.2004.12.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A derivatization reagent, 2-hydrazino-1-methylpyridine, was developed for the liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) of oxosteroids. The reagent quantitatively reacted with oxosteroids at 60 degrees C within 1 h and the resulting derivatives of the mono-oxosteroids provided a 70-1600-fold higher sensitivity compared to intact steroids. However, HMP was unsuitable for di-oxosteroids, such as androstenedione and progesterone. The developed derivatization procedure was applied to the LC-ESI-MS analysis of 5 alpha-dihydrotestosterone in human prostate, and allowed the reproducible quantification of nanogram/gram, level of the androgen with a 10-mg sample. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：214 / 222

页数：9

共 13 条

[1] SYNTHESIS OF TRI-DEUTERATED TESTOSTERONE LABELED SELECTIVELY AT C-19 ANGULAR METHYL-GROUP [J].