Comparison of media and conditions of incubation for the quantitative culture of Malassezia pachydermatis from canine skin

被引：16

作者：

Bond, R

Lloyd, DH

机构：

[1] Dept. of Small Anim. Med. and Surg., Royal Veterinary College, University of London, North Mymms, Hatfield, Herts AL9 7TA, Hawkshead lane

来源：

RESEARCH IN VETERINARY SCIENCE | 1996年 / 61卷 / 03期

关键词：

D O I：

10.1016/S0034-5288(96)90078-8

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Fifty-five swab-wash specimens from dogs were cultured at 32 degrees C on Sabouraud's dextrose agar either with or without 1 per cent Tween 80, Ushijima's medium A and modified Dixon's and Leeming's media. The counts of Malassezia pachydermatis were not significantly different after three or seven days of incubation. Colony counts on contact plates were significantly greater after incubation for seven days on Sabouraud's dextrose (P<0 . 001) and modified Dixon's agars (P<0 . 05) than after three days at 26 degrees C, but not at 32 degrees C. Counts on Sabouraud's or modified Dixon's agars after incubation at 32 degrees C and 37 degrees C were not significantly different. When compared with aerobic culture, an atmosphere containing 5 to 10 per cent carbon dioxide significantly (P<0 . 01) increased the frequency of isolation and colony counts on Sabouraud's dextrose agar but not on modified Dixon's agar.

引用

页码：273 / 274

页数：2

共 19 条

[11]

KORTING HC, 1991, MYCOSES, V34, P267, DOI 10.1111/j.1439-0507.1991.tb00657.x

[12] THE MICROBIAL ENVIRONMENT OF THE EAR CANAL IN HEALTH AND DISEASE [J].

KOWALSKI, JJ .

VETERINARY CLINICS OF NORTH AMERICA-SMALL ANIMAL PRACTICE, 1988, 18 (04) :743-754

[13] IMPROVED METHODS FOR ISOLATION AND ENUMERATION OF MALASSEZIA-FURFUR FROM HUMAN-SKIN [J].

LEEMING, JP ;

NOTMAN, FH .

JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (10) :2017-2019

[14] THE DIVERSITY OF PITYROSPORUM (MALASSEZIA) YEASTS INVIVO AND INVITRO [J].

MIDGLEY, G .

MYCOPATHOLOGIA, 1989, 106 (03) :143-153

[15]

NOBLE WC, 1974, MICROBIOLOGY HUMAN S, P316

[16] A SURVEY OF 120 ISOLATES OF MALASSEZIA (PITYROSPORUM) PACHYDERMATIS - PRELIMINARY-STUDY [J].

SANGUINETTI, V ;

TAMPIERI, MP ;

MORGANTI, L .

MYCOPATHOLOGIA, 1984, 85 (1-2) :93-95

[17] SELECTIVE AND DIFFERENTIAL MEDIA FOR ISOLATION AND TENTATIVE IDENTIFICATION OF EACH SPECIES OF PITYROSPORUM RESIDING ON NORMAL OR DISEASED HUMAN-SKIN [J].

USHIJIMA, T ;

TAKAHASHI, M ;

OZAKI, Y .

MICROBIOLOGY AND IMMUNOLOGY, 1981, 25 (11) :1109-1118

[18]

Van Abbe N.J., 1964, J SOC COSMET CHEM, V15, P609

[19]

Yarrow D., 1984, The yeasts. A taxonomic study, P882

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