Chronic ethanol on mRNA levels of IP(3)R1, IP3 3-kinase and mGluR1 in mouse Purkinje neurons

被引:29
作者
Simonyi, A
Zhang, JP
Sun, AY
Sun, GY
机构
[1] UNIV MISSOURI,DEPT BIOCHEM,COLUMBIA,MO 65212
[2] UNIV MISSOURI,DEPT PHARMACOL,COLUMBIA,MO 65212
关键词
alcoholism; Purkinje cells; in situ hybridization; inositol 1,4,5-trisphosphate receptor 1; inositol 1,4,5-trisphosphate 3-kinase; metabotropic glutamate receptor;
D O I
10.1097/00001756-199609020-00010
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
CHRONIC ethanol exposure is known to alter the polyphosphoinositide (poly-PI) signaling pathway, although the exact sight of regulation is not well understood. In this study, C57B1/6J mice were given a control or an ethanol (6 g kg(-1) body wt) liquid diet by gastric intubation daily for 4 weeks. Examination of levels of mRNA for type 1 inositol 1,4,5-trisphosphate receptor (IP(3)R1), inositol 1,4,5-trisphosphate 3-kinase (IP3K) and metabotropic glutamate receptor 1 (mGluR1) in cerebellar Purkinje neurons by in situ hybridization revealed significant decreases in cellular levels of mRNA encoding both IP(3)R1 and mGluR1 in the ethanol-treated group compared with controls, but levels of IP3K mRNA were not altered. These results suggest that chronic ethanol consumption selectively alters cellular mRNA in the poly-PI signaling pathway. The decrease in mRNA levels of mGluR1 and IP(3)R1 may be an important underlying mechanism towards explaining the alcohol-induced degenerative changes in the cerebellar Purkinje neurons.
引用
收藏
页码:2115 / 2118
页数:4
相关论文
共 20 条
[1]   INOSITOL TRISPHOSPHATE AND CALCIUM SIGNALING [J].
BERRIDGE, MJ .
NATURE, 1993, 361 (6410) :315-325
[2]   GLUTAMATE AND QUISQUALATE REGULATE EXPRESSION OF METABOTROPIC GLUTAMATE RECEPTOR MESSENGER-RNA IN CULTURED CEREBELLAR GRANULE CELLS [J].
BESSHO, Y ;
NAWA, H ;
NAKANISHI, S .
JOURNAL OF NEUROCHEMISTRY, 1993, 60 (01) :253-259
[3]   INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR-MEDIATED CA2+ SIGNALING IN THE BRAIN [J].
FURUICHI, T ;
MIKOSHIBA, K .
JOURNAL OF NEUROCHEMISTRY, 1995, 64 (03) :953-960
[4]   INFLUENCE OF ETHANOL ON CALCIUM, INOSITOL PHOSPHOLIPIDS AND INTRACELLULAR SIGNALING MECHANISMS [J].
GANDHI, CR ;
ROSS, DH .
EXPERIENTIA, 1989, 45 (05) :407-413
[5]   COMPARISON OF NEURONAL INOSITOL 1,4,5-TRISPHOSPHATE 3-KINASE AND RECEPTOR MESSENGER-RNA DISTRIBUTIONS IN THE ADULT-RAT BRAIN USING INSITU HYBRIDIZATION HISTOCHEMISTRY [J].
MAILLEUX, P ;
TAKAZAWA, K ;
ERNEUX, C ;
VANDERHAEGHEN, JJ .
NEUROSCIENCE, 1992, 49 (03) :577-590
[6]   INOSITOL 1,4,5-TRISPHOSPHATE 3-KINASE DISTRIBUTION IN THE RAT-BRAIN - HIGH-LEVELS IN THE HIPPOCAMPAL CA1 PYRAMIDAL AND CEREBELLAR PURKINJE-CELLS SUGGEST ITS INVOLVEMENT IN SOME MEMORY PROCESSES [J].
MAILLEUX, P ;
TAKAZAWA, K ;
ERNEUX, C ;
VANDERHAEGHEN, JJ .
BRAIN RESEARCH, 1991, 539 (02) :203-210
[7]   SEQUENCE AND EXPRESSION OF A METABOTROPIC GLUTAMATE RECEPTOR [J].
MASU, M ;
TANABE, Y ;
TSUCHIDA, K ;
SHIGEMOTO, R ;
NAKANISHI, S .
NATURE, 1991, 349 (6312) :760-765
[8]  
MIGNERY GA, 1990, J BIOL CHEM, V265, P12679
[9]  
PENTNEY RJ, 1993, ALCOHOL INDUCED BRAI, V22, P2249
[10]  
PIN JP, 1995, NEUROPHARMACOLOGY, V34, P1, DOI 10.1016/0028-3908(94)00129-G