Sensitive and Specific Modified Hodge Test for KPC and Metallo-Beta-Lactamase Detection in Pseudomonas aeruginosa by Use of a Novel Indicator Strain, Klebsiella pneumoniae ATCC 700603

被引:49
作者
Pasteran, Fernando [1 ]
Veliz, Omar [1 ]
Rapoport, Melina [1 ]
Guerriero, Leonor [1 ]
Corso, Alejandra [1 ]
机构
[1] INEI ANLIS Dr Carlos G Malbran, Serv Antimicrobianos, Minist Salud & Ambiente, Dept Bacteriol, Buenos Aires, DF, Argentina
关键词
D O I
10.1128/JCM.05602-11
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We evaluated the ability of the modified Hodge test to discriminate between KPC- and metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa isolates and carbapenemase nonproducers. With Escherichia coli ATCC 25922 as the indicator strain, the MHT resulted in low sensitivity, specificity, and repeatability. Replacing the indicator strain with Klebsiella pneumoniae ATCC 700603 led to an improved performance (100%, 97%, 0%, and 100% sensitivity, specificity, indeterminate results and repeatability, respectively).
引用
收藏
页码:4301 / 4303
页数:3
相关论文
共 7 条
[1]  
[Anonymous], 2011, Performance standards for antimicrobial disk susceptibility tests
[2]  
approved standard M2-A11, V11th
[3]   Controlling the spread of carbapenemase-producing Gram-negatives: therapeutic approach and infection control [J].
Carmeli, Y. ;
Akova, M. ;
Cornaglia, G. ;
Daikos, G. L. ;
Garau, J. ;
Harbarth, S. ;
Rossolini, G. M. ;
Souli, M. ;
Giamarellou, H. .
CLINICAL MICROBIOLOGY AND INFECTION, 2010, 16 (02) :102-111
[4]  
Correa A, 2011, 51 INT C ANT AG CHEM
[5]   Modified Hodge and EDTA-disk synergy tests to screen metallo-β-lactamase-producing strains of Pseudomonas and Acinetobacter species [J].
Lee, K ;
Chong, Y ;
Shin, HB ;
Kim, YA ;
Yong, D ;
Yum, JH .
CLINICAL MICROBIOLOGY AND INFECTION, 2001, 7 (02) :88-91
[6]   Acquired carbapenemases in Gram-negative bacterial pathogens: detection and surveillance issues [J].
Miriagou, V. ;
Cornaglia, G. ;
Edelstein, M. ;
Galani, I. ;
Giske, C. G. ;
Gniadkowski, M. ;
Malamou-Lada, E. ;
Martinez-Martinez, L. ;
Navarro, F. ;
Nordmann, P. ;
Peixe, L. ;
Pournaras, S. ;
Rossolini, G. M. ;
Tsakris, A. ;
Vatopoulos, A. ;
Canton, R. .
CLINICAL MICROBIOLOGY AND INFECTION, 2010, 16 (02) :112-122
[7]   A simple test for the detection of KPC and metallo-β-lactamase carbapenemase-producing Pseudomonas aeruginosa isolates with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin [J].
Pasteran, F. ;
Veliz, O. ;
Faccone, D. ;
Guerriero, L. ;
Rapoport, M. ;
Mendez, T. ;
Corso, A. .
CLINICAL MICROBIOLOGY AND INFECTION, 2011, 17 (09) :1438-1441