Characterization of endogenous avian leukosis viruses in chicken embryonic fibroblast substrates used in production of measles and mumps vaccines

被引:35
作者
Johnson, JA [1 ]
Heneine, W [1 ]
机构
[1] Ctr Dis Control & Prevent, HIV & Retrovirol Branch, Div AIDS STD & TB Lab Res, Natl Ctr Infect Dis, Atlanta, GA 30333 USA
关键词
D O I
10.1128/JVI.75.8.3605-3612.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Previous findings of low levels of reverse transcriptase (RT) activity in chick cell-derived measles and mumps vaccines showed this activity to be associated with virus particles containing RNA of both subgroup E endogenous avian leukosis viruses (ALV-E) and endogenous avian viruses (EAV). These particles originate from chicken embryonic fibroblast (CEF) substrates used for propagating vaccine strains. To better characterize vaccine-associated ALV-E, we examined the endogenous ALV proviruses (ev loci) present in a White Leghorn CEF substrate pool by restriction fragment length polymorphism. Five ev loci were detected, ev-1, ev-3, ev-6, ev-18, and ev-19. Both ev-18 and ev-19 can express infectious ALV-E, while ev-1, ev-3, and ev-6 are defective. We analyzed the full-length sequence of ev-1 and identified an adenosine insertion within the pol RT-beta region at position 5026, which results in a truncated RT-beta and integrase. We defined the 1,692-bp deletion in the gag-pol region of ev-3, and we found that in ev-6, sequences from the 5' long terminal repeat to the 5' pol region were absent. Based on the sequences of the ev loci, RT-PCR assays were developed to examine expression of ALV-E particles (EV) in CEF supernatants. Both ev-1- and ev-3-like RNA sequences were identified, as well as two other RNA sequences with intact pol regions, presumably of ev-18 and ev-19 origin. Inoculation of susceptible quail fibroblasts with CEF culture supernatants from both 5-azacytidine-induced and noninduced CEF led to ALV infection, confirming the presence of infectious ALV-E. Our data demonstrate that both defective and nondefective ev loci can be present in CEF vaccine substrates and suggest that both ev classes may contribute to the ALV present in vaccines.
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页码:3605 / 3612
页数:8
相关论文
共 45 条
[11]   ROLE OF METHYLATION IN THE INDUCED AND SPONTANEOUS EXPRESSION OF THE AVIAN ENDOGENOUS VIRUS EV-1 - DNA-STRUCTURE AND GENE-PRODUCTS [J].
CONKLIN, KF ;
COFFIN, JM ;
ROBINSON, HL ;
GROUDINE, M ;
EISENMAN, R .
MOLECULAR AND CELLULAR BIOLOGY, 1982, 2 (06) :638-652
[12]  
COOPER MD, 1968, JNCI-J NATL CANCER I, V41, P373
[13]  
Crittenden L. B., 1991, Critical Reviews in Poultry Biology, V3, P73
[14]  
CRITTENDEN LB, 1979, JNCI-J NATL CANCER I, V63, P191
[15]   LOW INCIDENCE OF LYMPHOID TUMORS IN CHICKENS CONTINUOUSLY PRODUCING ENDOGENOUS VIRUS [J].
CRITTENDEN, LB ;
WITTER, RL ;
FADLY, AM .
AVIAN DISEASES, 1979, 23 (03) :646-653
[16]   INDUCTION OF NEOPLASMS BY SUBGROUP-E RECOMBINANTS OF EXOGENOUS AND ENDOGENOUS AVIAN RETROVIRUSES (ROUS-ASSOCIATED VIRUS TYPE-60) [J].
CRITTENDEN, LB ;
HAYWARD, WS ;
HANAFUSA, H ;
FADLY, AM .
JOURNAL OF VIROLOGY, 1980, 33 (02) :915-919
[17]   INCIDENCE OF AVIAN-LEUKOSIS VIRUS-INFECTION IN BROILER STOCKS AND ITS EFFECT ON EARLY GROWTH [J].
CRITTENDEN, LB ;
OKAZAKI, W ;
SMITH, EJ .
POULTRY SCIENCE, 1983, 62 (12) :2383-2386
[18]   FUNCTIONAL-ANALYSIS OF THE TRANSCRIPTION CONTROL REGION LOCATED WITHIN THE AVIAN RETROVIRAL LONG TERMINAL REPEAT [J].
CULLEN, BR ;
RAYMOND, K ;
JU, G .
MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (03) :438-447
[19]   DETERMINANTS FOR RECEPTOR INTERACTION AND CELL KILLING ON THE AVIAN RETROVIRUS GLYCOPROTEIN GP85 [J].
DORNER, AJ ;
COFFIN, JM .
CELL, 1986, 45 (03) :365-374
[20]   CHRONIC MYOCARDITIS AND CIRCULATORY SYNDROME IN A WHITE LEGHORN STRAIN INDUCED BY AN AVIAN-LEUKOSIS VIRUS - LIGHT AND ELECTRON-MICROSCOPIC STUDY [J].
GILKA, F ;
SPENCER, JL .
AVIAN DISEASES, 1990, 34 (01) :174-184