Effect of wastewater composition on archaeal population diversity

被引:62
作者
Akarsubasi, AT
Ince, O
Kirdar, B
Oz, NA
Orhon, D
Curtis, TP
Head, IM
Ince, BK [1 ]
机构
[1] Bogazici Univ, Inst Environm Sci, TR-34342 Istanbul, Turkey
[2] Istanbul Tech Univ, Dept Environm Engn, TR-34468 Istanbul, Turkey
[3] Bogazici Univ, Dept Chem Engn, TR-34342 Istanbul, Turkey
[4] Newcastle Univ, Sch Civil Engn & Geosci, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
[5] Newcastle Univ, Ctr Mol Ecol, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
基金
英国工程与自然科学研究理事会;
关键词
anaerobic treatment; archaeal diversity; acetoclastic methanogenic activity; completely stirred tank reactor; pharmaceutical wastewater; 16S rRNA gene;
D O I
10.1016/j.watres.2004.12.041
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Distribution and occurrence of Archaea and methanogenic activity in a laboratory scale, completely mixed anaerobic reactor treating pharmaceutical wastewaters were investigated and associated with reactor performance. The reactor was initially seeded with anaerobic digester sludge from an alcohol distillery wastewater treatment plant and was subjected to a three step feeding strategy. The feeding procedure involved gradual transition from a glucose containing feed to a solvent stripped pharmaceutical wastewater and then raw pharmaceutical wastewater. During the start-up period, over 90 % COD removal efficiency at an organic loading rate (OLR) of 6 kg COD m(-3) d(-1) was achieved with glucose feeding, and acetoclastic methanogenic activity was 336 ml CH4 gTVS(-1) d(-1). At the end of the primary loading, when the feed contained solvent stripped pharmaceutical wastewater at full composition, 71 % soluble COD removal efficiency was obtained and acetoclastic methanogenic activity decreased to half of the rate under glucose feed (166 ml CH4 gTVS(-1) d(-1)). At the end of secondary loading with 60 % (w/v) raw pharmaceutical wastewater, COD removal dropped to zero and acetoclastic methanogenic activity fell to less than 10 ml CH4 gTVS(-1) d(-1). Throughout the course of the experiment, microbial community structure was monitored by DGGE analysis of 16S rRNA gene fragments. Five different archaeal taxa were identified and the predominant archaeal sequences belonged to methanogenic Archaea. Two of these showed greatest sequence identity with Methanobacterium formicicum and Methanosaeta concilii. The types of Archaea present changed little in response to changing feed composition but the relative contribution of different organisms identified in the archaeal DGGE profiles did change. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1576 / 1584
页数:9
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