Bacterial and archaeal 16S rDNA and 16S rRNA dynamics during an acetate crisis in an anaerobic digestor ecosystem

被引：142

作者：

Delbès, C ^{[1
]}

Moletta, R ^{[1
]}

Godon, JJ ^{[1
]}

机构：

[1] Inst Natl Rech Agron, Lab Biotechnol Environm, F-11100 Narbonne, France

来源：

FEMS MICROBIOLOGY ECOLOGY | 2001年 / 35卷 / 01期

关键词：

anaerobic digestion; imbalance; 16S ribosomal RNA; population dynamics; SSCP;

D O I：

10.1111/j.1574-6941.2001.tb00784.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The dynamics of bacterial and archaeal populations of a laboratory-scale anaerobic digestor were investigated during a crisis period of the process reflected by an accumulation of acetate. A culture-independent approach based on single strand conformation polymorphism (SSCP) analysis of total 16S rDNA and 16S rRNA amplification products was used. A spirochete and a Synergistes sp. showed high and changing activity levels during the study. A Clostridium sp, showed a transient increase in presence and activity concomitant with the highest acetate concentrations. A major shirt in the most active archaeal populations from hydrogenotrophic to acetoclastic methanogens preceded the recovery of the reactor. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：19 / 26

页数：8

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