Photo-cross-linked small-molecule affinity matrix for facilitating forward and reverse chemical genetics

被引：98

作者：

Kanoh, N ^{[1
]}

Honda, K ^{[1
]}

Simizu, S ^{[1
]}

Muroi, M ^{[1
]}

Osada, H ^{[1
]}

机构：

[1] RIKEN, Discovery Res Inst, Antibiot Lab, Wako, Saitama 3510198, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 23期

关键词：

affinity purification; immobilization; molecular targeting; peptides; photochemistry;

D O I：

10.1002/anie.200462370

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) A photoaffinity reaction has been used to photo-cross-link small molecules on agarose beads to identify and purify the binding proteins (see picture; PMF: peptide mass fingerprinting). This technology is considered potentially useful for the confirmation of specificity between a small molecule and a protein of interest in the field of reverse chemical genetics. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：3559 / 3562

页数：4

共 20 条

[1] Controlling programmed cell death with a cyclophilin-cyclosporin-based chemical inducer of dimerization [J].

Belshaw, PJ ;

Spencer, DM ;

Crabtree, GR ;

Schreiber, SL .

CHEMISTRY & BIOLOGY, 1996, 3 (09) :731-738

[2]

BRUNNER J, 1980, J BIOL CHEM, V255, P3313

[3] ANTIBODIES TO CYCLOSPORINE-A (CSA) BY A NOVEL ROUTE AND THEIR USE TO MONITOR CYCLOSPORINE LEVELS BY RADIOIMMUNOASSAY (RIA) [J].

CACALANO, NA ;

CLEVELAND, WL ;

ERLANGER, BF .

JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 118 (02) :257-263

[4] EVIDENCE FOR A FUNCTIONAL RECEPTOR FOR CYCLOSPORINE-A ON THE SURFACE F LYMPHOCYTES [J].

CACALANO, NA ;

CHEN, BX ;

CLEVELAND, WL ;

ERLANGER, BF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (10) :4353-4357

[5] SYNTHESIS OF THE MAIN METABOLITE (OL-17) OF CYCLOSPORIN-A [J].

EBERLE, MK ;

NUNINGER, F .

JOURNAL OF ORGANIC CHEMISTRY, 1992, 57 (09) :2689-2691

[6] CYCLOPHILIN - A SPECIFIC CYTOSOLIC BINDING-PROTEIN FOR CYCLOSPORIN-A [J].

HANDSCHUMACHER, RE ;

HARDING, MW ;

RICE, J ;

DRUGGE, RJ .

SCIENCE, 1984, 226 (4674) :544-547

[7] A RECEPTOR FOR THE IMMUNOSUPPRESSANT FK506 IS A CIS-TRANS PEPTIDYL-PROLYL ISOMERASE [J].

HARDING, MW ;

GALAT, A ;

UEHLING, DE ;

SCHREIBER, SL .

NATURE, 1989, 341 (6244) :758-760

[8] Immobilization of natural products on glass slides by using a photoaffinity reaction and the detection of protein-small-molecule interactions [J].

Kanoh, N ;

Kumashiro, S ;

Simizu, S ;

Kondoh, Y ;

Hatakeyama, S ;

Tashiro, H ;

Osada, H .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (45) :5584-5587

[9]

Kanoh N., 2003, ANGEW CHEM, V115, P5742

[10]

KANOH N, UNPUB

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