Thermodynamic stability of RNA structures formed by CNG trinucleotide repeats. Implication for prediction of RNA structure

被引：42

作者：

Broda, M ^{[1
]}

Kierzek, E ^{[1
]}

Gdaniec, Z ^{[1
]}

Kulinski, T ^{[1
]}

Kierzek, R ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Bioorgan Chem, PL-61704 Poznan, Poland

来源：

BIOCHEMISTRY | 2005年 / 44卷 / 32期

关键词：

D O I：

10.1021/bi0502339

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Trinucleotide repeat expansion diseases (TREDs) are correlated with elongation of CNG DNA and RNA repeats to pathological level. This paper shows, for the first time, complete data concerning thermodynamic stabilities of RNA with CNG trinucleotide repeats. Our studies include the stability of oligoribonucleotides composed of two to seven of CAG, CCG, CGG, and CUG repeats. The thermodynamic parameters of helix propagation correlated with the presence of multiple N-N mismatches within CNG RNA duplexes were also determined. Moreover, the total stability of CNG RNA hairpins, as well as the contribution of trinucleotide repeats placed only in the stem or loop regions, was evaluated. The improved thermodynamic parameters allow to predict much more accurately the thermodynamic stabilities and structures of CNG RNAs.

引用

页码：10873 / 10882

页数：10

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