Deletion of Munc18-1 in 5-HT Neurons Results in Rapid Degeneration of the 5-HT System and Early Postnatal Lethality

被引：11

作者：

Dudok, Jacobus J. ^{[1
]}

Groffen, Alexander J. A. ^{[1
]}

Toonen, Ruud F. T. ^{[1
]}

Verhage, Matthijs ^{[1
]}

机构：

[1] Vrije Univ Amsterdam, Dept Funct Genom, CNCR, Amsterdam, Netherlands

来源：

PLOS ONE | 2011年 / 6卷 / 11期

关键词：

SEROTONIN NEURONS; REGULATED SECRETION; BRAIN-SEROTONIN; KNOCKOUT MICE; ABSENCE; INNERVATION;

D O I：

10.1371/journal.pone.0028137

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The serotonin (5-HT) system densely innervates many brain areas and is important for proper brain development. To specifically ablate the 5-HT system we generated mutant mice carrying a floxed Munc18-1 gene and Cre recombinase driven by the 5-HT-specific serotonin reuptake transporter (SERT) promoter. The majority of mutant mice died within a few days after birth. Immunohistochemical analysis of brains of these mice showed that initially 5-HT neurons are formed and the cortex is innervated with 5-HT projections. From embryonic day 16 onwards, however, 5-HT neurons started to degenerate and at postnatal day 2 hardly any 5-HT projections were present in the cortex. The 5-HT system of mice heterozygous for the floxed Munc18-1 allele was indistinguishable from control mice. These data show that deletion of Munc18-1 in 5-HT neurons results in rapid degeneration of the 5-HT system and suggests that the 5-HT system is important for postnatal survival.

引用

页数：6

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