Apple (Malus pumila) procyanidins fractionated according to the degree of polymerization using normal-phase chromatography and characterized by HPLC-ESI/MS and MALDI-TOF/MS

被引：81

作者：

Shoji, T ^{[1
]}

Masumoto, S ^{[1
]}

Moriichi, N ^{[1
]}

Kanda, T ^{[1
]}

Ohtake, Y ^{[1
]}

机构：

[1] Asahi Brewery Co Ltd, Fundamental Res Lab, Moriya, Ibaraki 3020106, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1102卷 / 1-2期

关键词：

apple procyanidins; doubly charged ions; HPLC-ESI/MS; MALDI-TOF/MS; normal-phase chromatography;

D O I：

10.1016/j.chroma.2005.10.065

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Our previously reported method for the fractionation of apple procyanidins was modified successfully to achieve the separation of (epi)catechins and procyanidins (ranging from dimers to octamers) according to the degree of polymerization. Normal-phase chromatography was employed, using a hexane-methanol-ethyl acetate mixture as the mobile phase. Each fraction was characterized using high-performance liquid-chromatography electrospray-ionization mass spectrometry (HPLC-ESI/MS) and matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF/MS). This method will be useful for the evaluation of the physiological functions of proanthocyanidins and for the elucidation of their individual structures. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：206 / 213

页数：8

共 58 条

[1]

Akiyama H, 2000, BIOL PHARM BULL, V23, P1370, DOI 10.1248/bpb.23.1370

[2] ANTIOXIDATIVE PROPERTIES OF PROANTHOCYANIDINS .1. ANTIOXIDATIVE PROPERTIES OF PROCYANIDIN-B-1 AND PROCYANIDIN-B-3 FROM AZUKI BEANS IN AQUEOUS SYSTEMS [J].

ARIGA, T ;

KOSHIYAMA, I ;

FUKUSHIMA, D .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1988, 52 (11) :2717-2722

[3]

Cheynier V, 1999, METHOD ENZYMOL, V299, P178

[4]

Dadic M., 1976, Journal of the American Society of Brewing Chemists, V34, P158

[5]

de Gaulejac NSC, 1999, J SCI FOOD AGR, V79, P1081, DOI 10.1002/(SICI)1097-0010(199906)79:8&lt

[6]

1081::AID-JSFA330&gt

[7]

3.0.CO

[8]

2-G

[9]

DERDELINCKX G, 1994, J CHROMATOGR, V285, P231

[10]

FOO LY, 1991, PHYTOCHEMISTRY, V30, P667, DOI 10.1016/0031-9422(91)83749-B

← 1 2 3 4 5 6 →