Detection of Treponema denticola in atherosclerotic lesions

被引：86

作者：

Okuda, K

Ishihara, K

Nakagawa, T

Hirayama, A

Inayama, Y

Okuda, K

机构：

[1] Tokyo Dent Coll, Dept Microbiol, Mihama Ku, Chiba, Japan

[2] Tokyo Dent Coll, Dept Periodont, Chiba, Japan

[3] Tokyo Dent Coll, Oral Hlth Sci Ctr, Chiba, Japan

[4] Yokohama City Univ, Sch Med, Dept Bacteriol, Yokohama, Kanagawa 232, Japan

[5] Yokohama City Univ, Sch Med, Dept Anat & Surg Pathol, Yokohama, Kanagawa 232, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 03期

关键词：

D O I：

10.1128/JCM.39.3.1114-1117.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We examined 26 atherosclerotic lesions and 14 nondiseased aorta specimens to detect the periodontopathogenic part of the bacterial 16S rRNA locus by PCR. Treponema denticola sequence of the 16S rRNA locus was found in 6 out of 26 DNA samples (23.1%) from the formalin-fixed, paraffin-embeded atherosclerotic lesions obtained during surgery but not in any of the 14 nondiseased aorta samples from deceased persons. Utilizing immunofluorescence microscopy, we observed aggregated antigenic particles reacting with rabbit antiserum against T. denticola in thin sections of the PCR-positive samples, but we could not detect any reacting particles in the PCR-negative thin sections.

引用

页码：1114 / 1117

页数：4

共 29 条

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