Identification and characterisation of a new human glucose-6-phosphatase isoform

被引：71

作者：

Guionie, O

Clottes, E

Stafford, K

Burchell, A ^{[1
]}

机构：

[1] Univ Dundee, Ninewells Hosp & Med Sch, Dundee DD1 9SY, Scotland

[2] Fac Med, Lab Inter Univ Act Phys & Sport, F-63001 Clermont Ferrand, France

来源：

FEBS LETTERS | 2003年 / 551卷 / 1-3期

基金：

英国惠康基金;

关键词：

brain; liver; glucose-6-phosphatase; enzymatic activity;

D O I：

10.1016/S0014-5793(03)00903-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The liver endoplasmic reticulum glucose-6-phosphatase catalytic subunit (G6PC1) catalyses glucose 6-phosphate hydrolysis during gluconeogenesis and glycogenolysis. The highest glucose-6-phosphatase activities are found in the liver and the kidney; there have been many reports of glucose 6-phosphate hydrolysis in other tissues. We cloned a new G6Pase isoform (G6PC3) from human brain encoded by a six-exon gene (chromosome 17q21). G6PC3 protein was able to hydrolyse glucose 6-phosphate in transfected Chinese hamster ovary cells. The optimal pH for glucose 6-phosphate hydrolysis was lower and the K. higher relative to G6PC1. G6PC3 preferentially hydrolyzed other substrates including pNPP and 2-deoxy-glucose-6-phosphate compared to the liver enzyme. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：159 / 164

页数：6

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