Self-gelatinizable copolymer immobilized glucose biosensor based on Prussian Blue modified graphite electrode

被引:38
作者
Deng, Q [1 ]
Li, B [1 ]
Dong, SJ [1 ]
机构
[1] Chinese Acad Sci, Changchun Inst Appl Chem, Electroanalyt Chem Lab, Changchun 130022, Peoples R China
关键词
D O I
10.1039/a803309i
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel poly(vinyl alcohol) grafting 4-vinylpyridine self-gelatinizable copolymer was adapted to immobilize glucose oxidase. The reduction of hydrogen peroxide (H2O2) was detected at a Prussian Blue (PB) modified graphite electrode. A stable and sensitive glucose amperometric biosensor is described. The copolymer is a good biocompatible polymer in which the glucose oxidase retains high activity. Moreover, the copolymer can adhere firmly to the inorganic PB membrane. The sensor showed an apparent Michaelis-Menten constant of 18 +/- 0.2 mM and a maximum current density of 1.14 mu A cm(-2) mM(-1). The linear range is from 5 mu M to 4.5 mM glucose and the detection limit is 0.5. mu M glucose. The catalytic efficiency of PB for the reduction of H2O2 is higher than that for the oxidation of H2O2. Glucose concentrations in serum samples from healthy persons and diabetic patients were determined using the sensor. The results compared well with those provided by the hospital using a spectroscopy method.
引用
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页码:1995 / 1999
页数:5
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