Siroheme biosynthesis in Saccharomyces cerevisiae requires the products of both the MET1 and MET8 genes

被引：30

作者：

Hansen, J ^{[1
]}

Muldbjerg, M ^{[1
]}

Cherest, H ^{[1
]}

SurdinKerjan, Y ^{[1
]}

机构：

[1] CNRS, CTR GENET MOL, F-91198 GIF SUR YVETTE, FRANCE

来源：

FEBS LETTERS | 1997年 / 401卷 / 01期

关键词：

siroheme biosynthesis; yeast;

D O I：

10.1016/S0014-5793(96)01423-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Siroheme is a uroporphyrinogen III-derivative used by sulfite reductase as a prosthetic group. We investigated in Saccharomyces cerevisiae the possible involvement in siroheme biosynthesis of three genes, MET1, MET8 and MET20. The MET1 gene from S. cerevisiae mas cloned and shown to be the same gene as MET20. Sequence similitudes as well as complementation studies indicate that Met1p and Met8p are both involved in siroheme biosynthesis. In addition, we show formally that S. cerevisiae does not need vitamin B-12 for growth.

引用

页码：20 / 24

页数：5

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