2 DIVERGENT MET10 GENES, ONE FROM SACCHAROMYCES-CEREVISIAE AND ONE FROM SACCHAROMYCES-CARLSBERGENSIS, ENCODE THE ALPHA-SUBUNIT OF SULFITE REDUCTASE AND SPECIFY POTENTIAL BINDING-SITES FOR FAD AND NADPH

被引:44
作者
HANSEN, J
CHEREST, H
KIELLANDBRANDT, MC
机构
[1] CARLSBERG LAB,DEPT YEAST GENET,DK-2500 COPENHAGEN,DENMARK
[2] CNRS,CTR GENET MOLEC,GIF SUR YVETTE,FRANCE
关键词
D O I
10.1128/jb.176.19.6050-6058.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The yeast assimilatory sulfite reductase is a complex enzyme that is responsible for conversion of sulfite into sulfide. To obtain information on the nature of this enzyme, we isolated and sequenced the MET10 gene of Saccharomyces cerevisiae and a divergent MET10 allele from Saccharomyces carlsbergensis. The polypeptides deduced from the identically sized open reading frames (1,035 amino acids) of both MET10 genes have molecular masses of around 115 kDa and are 88% identical to each other. The transcript of S. cerevisiae MET10 has a size comparable to that of the open reading frame and is transcriptionally repressed by methionine in a way similar to that seen for other MET genes of S. cerevisiae. Distinct homology was found between the putative MET10-encoded polypeptide and flavin interacting parts of the sulfite reductase flavoprotein subunit (encoded by cysJ) from Escherichia coli and several other flavoproteins. A significant N-terminal homology to pyruvate flavodoxin oxidoreductase (encoded by nifJ) from Klebsiella pneumoniae, together with a lack of obvious flavin mononucleotide-binding motifs in the MET10 deduced amino acid sequence, suggests that the yeast assimilatory sulfite reductase is a distinct type of sulfite reductase.
引用
收藏
页码:6050 / 6058
页数:9
相关论文
共 62 条
[1]   GCN4 PROTEIN, A POSITIVE TRANSCRIPTION FACTOR IN YEAST, BINDS GENERAL CONTROL PROMOTERS AT ALL 5' TGACTC 3' SEQUENCES [J].
ARNDT, K ;
FINK, GR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (22) :8516-8520
[2]   PLASMIDS PEMBLY - NEW SINGLE-STRANDED SHUTTLE VECTORS FOR THE RECOVERY AND ANALYSIS OF YEAST DNA-SEQUENCES [J].
BALDARI, C ;
CESARENI, G .
GENE, 1985, 35 (1-2) :27-32
[3]   MOLECULAR-CLONING AND REGULATION OF THE EXPRESSION OF THE MET2 GENE OF SACCHAROMYCES-CEREVISIAE [J].
BARONI, M ;
LIVIAN, S ;
MARTEGANI, E ;
ALBERGHINA, L .
GENE, 1986, 46 (01) :71-78
[4]  
BENNETZEN JL, 1982, J BIOL CHEM, V257, P3026
[5]   STERILE HOST YEASTS (SHY) - EUKARYOTIC SYSTEM OF BIOLOGICAL CONTAINMENT FOR RECOMBINANT DNA EXPERIMENTS [J].
BOTSTEIN, D ;
FALCO, SC ;
STEWART, SE ;
BRENNAN, M ;
SCHERER, S ;
STINCHCOMB, DT ;
STRUHL, K ;
DAVIS, RW .
GENE, 1979, 8 (01) :17-24
[6]   CLONED AND EXPRESSED NITRIC-OXIDE SYNTHASE STRUCTURALLY RESEMBLES CYTOCHROME-P-450 REDUCTASE [J].
BREDT, DS ;
HWANG, PM ;
GLATT, CE ;
LOWENSTEIN, C ;
REED, RR ;
SNYDER, SH .
NATURE, 1991, 351 (6329) :714-718
[7]   THE NUCLEOTIDE-SEQUENCE OF THE NIFJ GENE OF KLEBSIELLA-PNEUMONIAE [J].
CANNON, M ;
CANNON, F ;
BUCHANANWOLLASTON, V ;
ALLY, D ;
ALLY, A ;
BEYNON, J .
NUCLEIC ACIDS RESEARCH, 1988, 16 (23) :11379-11379
[8]   CLONING AND ANALYSIS OF 2 ALLELES OF THE ILV3 GENE FROM SACCHAROMYCES CARLSBERGENSIS [J].
CASEY, GP .
CARLSBERG RESEARCH COMMUNICATIONS, 1986, 51 (05) :327-341
[9]  
CHEREST H, 1985, GENE, V34, P269
[10]   NUCLEOTIDE-SEQUENCE OF THE MET8 GENE OF SACCHAROMYCES-CEREVISIAE [J].
CHEREST, H ;
THOMAS, D ;
SURDINKERJAN, Y .
NUCLEIC ACIDS RESEARCH, 1990, 18 (03) :659-659