Self-assembled, mesoporous polymeric networks for patterned protein arrays

被引:12
作者
Batra, D
Vogt, S
Laible, PD
Firestone, MA
机构
[1] Argonne Natl Lab, Div Sci Mat, Ctr Nanoscale Mat, Expt Facil, Argonne, IL 60439 USA
[2] Argonne Natl Lab, Biosci Div, Argonne, IL 60439 USA
关键词
D O I
10.1021/la051948f
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A facile, self-assembly approach to the fabrication of a robust, mesoporous, biocompatible polymeric network for the spatial organization of proteins is described. Surface-deposited poly(styrene) (PS) beads that assemble into a two-dimensional (2-D) hexagonal array are used to template cross-linked poly(vinyl alcohol) (PVA), yielding an inverse opal structure. The porous, water insoluble network is used to entrain a model, soluble protein, green fluorescent protein (GFP). The polymeric network is characterized by atomic force microscopy (AFM) and optical microscopy, and the spatial localization of the incorporated GFP is determined by fluorescence microscopy. The results demonstrate that this system may constitute a versatile platform for the lateral organization of biomolecules.
引用
收藏
页码:10301 / 10306
页数:6
相关论文
共 28 条
[1]   THE SYNTHESIS OF SOME WATER INSOLUBLE DYES FOR THE MEASUREMENT OF PH IN WATER IMMISCIBLE SOLVENTS [J].
BROWN, L ;
HALLING, PJ ;
JOHNSTON, GA ;
SUCKLING, CJ ;
VALIVETY, RH .
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1990, (12) :3349-3353
[2]   Conversion of colloidal crystals to polymer nets: Turning latex particles inside out [J].
Chen, YY ;
Ford, WT ;
Materer, NF ;
Teeters, D .
CHEMISTRY OF MATERIALS, 2001, 13 (08) :2697-2704
[3]   Green fluorescent proteins as optically controllable elements in bioelectronics [J].
Cinelli, RAG ;
Pellegrini, V ;
Ferrari, A ;
Faraci, P ;
Nifosì, R ;
Tyagi, M ;
Giacca, M ;
Beltram, F .
APPLIED PHYSICS LETTERS, 2001, 79 (20) :3353-3355
[4]   Acid denaturation and refolding of green fluorescent protein [J].
Enoki, S ;
Saeki, K ;
Maki, K ;
Kuwajima, K .
BIOCHEMISTRY, 2004, 43 (44) :14238-14248
[5]   Rapid and high-capacity immobilization of enzymes based on mesoporous silicas with controlled morphologies [J].
Fan, J ;
Lei, J ;
Wang, LM ;
Yu, CZ ;
Tu, B ;
Zhao, DY .
CHEMICAL COMMUNICATIONS, 2003, (17) :2140-2141
[6]   Alignment control of a cyanine dye using a mesoporous silica film with uniaxially aligned mesochannels [J].
Fukuoka, A ;
Miyata, H ;
Kuroda, K .
CHEMICAL COMMUNICATIONS, 2003, (02) :284-285
[7]  
Hassan CM, 2000, ADV POLYM SCI, V153, P37
[8]   Design of photoresists with reduced environmental impact.: 1.: Water-soluble resists based on photo-cross-linking of poly(vinyl alcohol) [J].
Havard, JM ;
Shim, SY ;
Fréchet, JMJ ;
Lin, QH ;
Medeiros, DR ;
Willson, CG ;
Byers, JD .
CHEMISTRY OF MATERIALS, 1999, 11 (03) :719-725
[9]   Large-scale fabrication of wafer-size colloidal crystals, macroporous polymers and nanocomposites by spin-coating [J].
Jiang, P ;
McFarland, MJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (42) :13778-13786
[10]   Direct Patterning of protein- and cell-resistant polymeric monolayers and microstructures [J].
Khademhosseini, A ;
Jon, S ;
Suh, KY ;
Tran, TNT ;
Eng, G ;
Yeh, J ;
Seong, J ;
Langer, R .
ADVANCED MATERIALS, 2003, 15 (23) :1995-2000