Self-assembled, mesoporous polymeric networks for patterned protein arrays

被引：12

作者：

Batra, D

Vogt, S

Laible, PD

Firestone, MA

机构：

[1] Argonne Natl Lab, Div Sci Mat, Ctr Nanoscale Mat, Expt Facil, Argonne, IL 60439 USA

[2] Argonne Natl Lab, Biosci Div, Argonne, IL 60439 USA

来源：

LANGMUIR | 2005年 / 21卷 / 23期

关键词：

D O I：

10.1021/la051948f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A facile, self-assembly approach to the fabrication of a robust, mesoporous, biocompatible polymeric network for the spatial organization of proteins is described. Surface-deposited poly(styrene) (PS) beads that assemble into a two-dimensional (2-D) hexagonal array are used to template cross-linked poly(vinyl alcohol) (PVA), yielding an inverse opal structure. The porous, water insoluble network is used to entrain a model, soluble protein, green fluorescent protein (GFP). The polymeric network is characterized by atomic force microscopy (AFM) and optical microscopy, and the spatial localization of the incorporated GFP is determined by fluorescence microscopy. The results demonstrate that this system may constitute a versatile platform for the lateral organization of biomolecules.

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页码：10301 / 10306

页数：6

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