Tracing of axonal connectivities in a combined slice preparation of rat brains - a study by rhodamine-dextran-amine-application in the lateral nucleus of the amygdala

被引:30
作者
von Bohlen und Halbach, O [1 ]
Albrecht, D [1 ]
机构
[1] Humboldt Univ, Fac Med Charite, Inst Physiol, D-10117 Berlin, Germany
关键词
in-vitro; slice preparation; silver staining; fluorescent tracers; electrophysiology;
D O I
10.1016/S0165-0270(98)00026-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In preparation for electrophysiological studies? the fiber pathways within the amygdala of rats have been examined using a combined Nissl- and silver-staining method (Bielshowsky) in horizontal sections of the amygdala. To determine if fibers in the lateral nucleus of the amygdala are in functional connection with other brain regions, an anterograde and retrograde axonal tracing method (application of rhodamine-dextran-amine) was performed. Horizontal slices containing some nuclei of the amygdala (lateral nucleus, basolateral nucleus, central nucleus, medial nucleus), as well as the entorhinal cortex, the hippocampus and parts of the piriform cortex were used. Crystals of rhodemine-dextran-amine were placed in the lateral nucleus. Intra-amygdaloid connections as well as connections to other brain regions were preserved in this preparation. The connectivities of the lateral nucleus with other nuclei of the amygdala (basolateral, medial, central) correspond with the findings made by tracer applications in-vivo. The use of a tracer that is transported in both the antero- and retrograde direction shows the whole spectrum of efferent and afferent fibers which are involved in the projection pattern of the lateral nucleus. To test the usefulness of this preparation for electrophysiological studies, extracellular field potentials were recorded. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:169 / 175
页数:7
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