Maternal Circulating CD34+VEGFR-2+ and CD133+VEGFR-2+ Progenitor Cells Increase During Normal Pregnancy but Are Reduced in Women With Preeclampsia

被引：56

作者：

Luppi, Patrizia ^{[2
]}

Powers, Robert W. ^{[1
,3
]}

Verma, Vivek ^{[1
]}

Edmunds, Lia

Plymire, Daniel ^{[1
]}

Hubel, Carl A. ^{[1
,3
,4
,5
]}

机构：

[1] Magee Womens Res Inst, Pittsburgh, PA 15213 USA

[2] Dept Pediat, Pittsburgh, PA USA

[3] Dept Obstet Gynecol & Reprod Sci, Pittsburgh, PA USA

[4] Univ Pittsburgh, Grad Sch Publ Hlth, Dept Environm & Occupat Hlth, Pittsburgh, PA 15261 USA

[5] Univ Pittsburgh, Ctr Vasc Remo Deling & Regenerat, Pittsburgh, PA USA

来源：

REPRODUCTIVE SCIENCES | 2010年 / 17卷 / 07期

基金：

美国国家卫生研究院;

关键词：

Endothelial progenitor cells; pregnancy; preeclampsia; angiogenic factors; ENDOTHELIAL-CELLS; PERIPHERAL-BLOOD; TYROSINE KINASE-1; HYPERTENSION; NEOVASCULARIZATION; SUBPOPULATIONS; DYSFUNCTION; INSIGHTS; IDENTITY; STEM;

D O I：

10.1177/1933719110366164

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Circulating endothelial progenitor cells (EPCs) may contribute to vascular endothelial cell homeostasis, and low levels of these cells are predictive of cardiovascular disease. We hypothesized that circulating EPCs increase in number during uncomplicated pregnancy but are reduced in women with preeclampsia. Peripheral blood was obtained from pregnant women and from nulligravidas in cross-sectional design. Cells expressing CD34 or CD133, in combination with vascular endothelial growth factor receptor-2 (VEGFR-2), were enumerated by flow cytometry. Both CD34(+)VEGFR-2(+) (doubly positive) and CD133(+)VEGFR-2(+) cells were significantly increased during the second and third trimesters of uncomplicated pregnancy compared to the first trimester. First trimester and nulligravida groups did not differ. Endothelial progenitor cells, quantified by flow cytometry or by circulating angiogenic cell (CAC) culture assay, were significantly reduced in women with preeclampsia compared to third trimester controls. Circulating EPCs appear to increase during normal pregnancy, and comparatively reduced numbers of these cells exist during preeclampsia.

引用

页码：643 / 652

页数：10

共 38 条

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