A novel, efficient regenerating method of NADPH using a new formate dehydrogenase

被引：111

作者：

Seelbach, K

Riebel, B

Hummel, W

Kula, MR

Tishkov, VI

Egorov, AM

Wandrey, C

Kragl, U

机构：

[1] FORSCHUNGSZENTRUM JULICH, FORSCHUNGSZENTRUM, INST BIOTECHNOL, D-52425 JULICH, GERMANY

[2] UNIV DUSSELDORF, FORSCH ZENTRUM JULICH GMBH, INST ENZYMTECHNOL, D-52404 JULICH, GERMANY

[3] MOSCOW MV LOMONOSOV STATE UNIV, FAC CHEM, DEPT CHEM ENZYMOL, MOSCOW 119899, RUSSIA

来源：

TETRAHEDRON LETTERS | 1996年 / 37卷 / 09期

关键词：

D O I：

10.1016/0040-4039(96)00010-X

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A NADPH regenerating system using a new, protein engineered formate dehydrogenase (FDH) is investigated. The new enzyme is the first known NAD(P)H dependent FDH. It can be successfully employed in synthesis viith other enzymes requiring a NADPH regeneration. All advantages of the known NAD(H) dependent FDH's in these enzyme coupled synthesis can now be transferred to NADP(H) dependent systems.

引用

收藏

页码：1377 / 1380

页数：4

相关论文

共 25 条

[1] REGENERATION OF NICOTINAMIDE COFACTORS FOR USE IN ORGANIC-SYNTHESIS [J].

CHENAULT, HK ;

WHITESIDES, GM .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1987, 14 (02) :147-197

[2] NAD-DEPENDENT FORMATE DEHYDROGENASE FROM METHYLOTROPHIC BACTERIUM, STRAIN-1 - PURIFICATION AND CHARACTERIZATION [J].

EGOROV, AM ;

AVILOVA, TV ;

DIKOV, MM ;

POPOV, VO ;

RODIONOV, YV ;

BEREZIN, IV .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1979, 99 (03) :569-576

[3] ELECTROENZYMATIC SYNTHESIS (REGENERATION OF NADH COENZYME) - USE OF NAFION ION-EXCHANGE FILMS FOR IMMOBILIZATION OF ENZYME AND REDOX MEDIATOR [J].

FRY, AJ ;

SOBOLOV, SB ;

LEONIDA, MD ;

VOIVODOV, KI .

TETRAHEDRON LETTERS, 1994, 35 (31) :5607-5610

[4]

HUMMEL W, 1990, APPL MICROBIOL BIOT, V34, P15, DOI 10.1007/BF00170916

[5] ENZYME-CATALYZED SYNTHESIS OF OPTICALLY PURE R(+)-PHENYLETHANOL [J].

HUMMEL, W .

BIOTECHNOLOGY LETTERS, 1990, 12 (06) :403-408

[6] SYNTHETIC APPLICATIONS OF ALCOHOL-DEHYDROGENASE FROM THERMOANAEROBIUM-BROCKII [J].

KEINAN, E ;

SETH, KK ;

LAMED, R .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1987, 501 :130-149

[7] THERMOSTABLE ENZYMES IN ORGANIC-SYNTHESIS .2. ASYMMETRIC REDUCTION OF KETONES WITH ALCOHOL-DEHYDROGENASE FROM THERMOANAEROBIUM-BROCKII [J].

KEINAN, E ;

HAFELI, EK ;

SETH, KK ;

LAMED, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1986, 108 (01) :162-169

[8] NAD(P)H REGENERATION USING ETHANOL AS AN ENERGY-SOURCE IN BAKERS YEAST-MEDIATED BIOREDUCTION [J].

KOMETANI, T ;

MORITA, Y ;

FURUI, H ;

YOSHII, H ;

MATSUNO, R .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1994, 77 (01) :13-16

[9] ENZYMATIC 2-STEP SYNTHESIS OF N-ACETYLNEURAMINIC ACID IN THE ENZYME MEMBRANE REACTOR [J].

KRAGL, U ;

GYGAX, D ;

GHISALBA, O ;

WANDREY, C .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1991, 30 (07) :827-828

[10]

KRAGL U, IN PRESS BIOTECH BIO