Real-time monitoring approach: Assessment of effects of antibodies on the adhesion of NCI-H460 cancer cells to the extracellular matrix

被引:18
作者
Chen, Yu [1 ]
Zhang, Juntao [2 ]
Wang, Yanping [1 ]
Zhang, Li [1 ]
Julien, Reboud [1 ]
Tang, Kumcheong [1 ]
Balasubramanian, Narayanan [1 ]
机构
[1] Inst Microelect, Singapore 117685, Singapore
[2] Nanyang Technol Univ, Sch Chem & Biomed Engn, Singapore, Singapore
关键词
real-time cell-on-chip system; in situ cell monitoring; NCI-H460; adhesion; collagen-coated electrodes; alpha; 2; beta; 1-integrin;
D O I
10.1016/j.bios.2007.12.006
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
This paper presents an in situ impedance chip system, which allows studying the effects of drugs on the behaviors of living cell in real-time. A new label-free measurement approach is introduced, which enables to assess the extent of the adhesion of a cell population to the extracellular matrix (ECM)-coated rnicroelectrode array, by adding together all the impedance changes from individually controlled microelectrodes. A high sensitivity was demonstrated and allowed us to monitor cell attachment with the resolution of a "few cells". The dose and pre-incubation time effects of antibodies against beta 1-integrin and its subunit alpha 2 beta 1-integrin on the adhesion behavior of NCI-H460 lung cancer cells to collagen type I was extensively studied. Results show that both anti-beta 1-integrin and anti-alpha 2 beta 1-integrin inhibit NCI-H460 cells attachment to collagen I. This indicates that beta 1-integrin is present on the surface of NCI-H460 cancer cells, and that its subunit alpha 2 beta 1 significantly affects NCI-H460 cells attachment on collagen I. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:1390 / 1396
页数:7
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