Identifying the Early Post-HIV Antibody Seroconversion Period

被引：13

作者：

Hecht, Frederick M. ^{[1
]}

Wellman, Robert ^{[2
,3
]}

Busch, Michael P. ^{[2
]}

Pilcher, Christopher D.

Norris, Philip J. ^{[4
]}

Margolick, Joseph B. ^{[5
]}

Collier, Ann C. ^{[2
]}

Little, Susan J. ^{[6
]}

Markowitz, Martin ^{[7
]}

Routy, Jean-Pierre ^{[8
]}

Holte, Sarah ^{[2
,9
]}

机构：

[1] Univ Calif San Francisco, Div HIV AIDS, Ward 84, San Francisco, CA 94110 USA

[2] Univ Washington, Seattle, WA 98195 USA

[3] Grp Hlth Res Inst, Seattle, WA USA

[4] Blood Syst Res Inst, San Francisco, CA USA

[5] Johns Hopkins Bloomberg Sch Publ Hlth, Baltimore, MD USA

[6] Univ Calif San Diego, San Diego, CA 92103 USA

[7] Aaron Diamond AIDS Res Ctr, New York, NY USA

[8] McGill Univ, Ctr Hlth, Montreal, PQ, Canada

[9] Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA

来源：

JOURNAL OF INFECTIOUS DISEASES | 2011年 / 204卷 / 04期

关键词：

ANTIRETROVIRAL THERAPY; SET-POINT; INFECTION; VIRUS; IDENTIFICATION; IMMUNOASSAY; SYMPTOMS;

D O I：

10.1093/infdis/jir304

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background. Identifying persons with recent human immunodeficiency virus (HIV) antibody seroconversion is useful for treatment, research, and prevention, but the sensitivity and specificity of tests for this purpose are uncertain. Methods. We used longitudinal specimens panels from 155 persons identified prior to HIV seroconversion to assess antibody-based methods for classifying persons as within 30, 60, or 90 days of seroconversion, including 2 incidence assays, a less-sensitive (LS) enzyme immunoassay (EIA), and the BED assay. Results. Sensitivity and specificity, respectively, for identifying persons within 30 days of seroconversion were: 34%-57% and 98%-100% for 2 standard EIAs (employing a signal-to-cutoff < 4.0; >= 1.0 defines HIV positive), 84% and 73% for the LS-EIA (< 0.2 cutoff), 88% and 72% for the BED (< 0.2 cutoff), and 43%-58% and 98% (< 3 bands) for 2 Western blot (WB) assays. By area under the receiver operator curves, the best test for identifying persons within 30 days of seroconversion was the number of bands on the Bio-Rad WB (0.90); within 60 days, the LS-EIA and BED (both 0.85); and for persons within 90 days the BED (0.86). Conclusions. Standard EIAs, Western blots, and HIV incidence assays provide useful information for identifying persons 30 to 90 days after seroconversion.

引用

页码：526 / 533

页数：8