Trafficking of the Igα/Igβ heterodimer with membrane Ig and bound antigen to the major histocompatibility complex class II peptide-loading compartment

被引:13
作者
Brown, BK
Li, C
Cheng, PC
Song, WX [1 ]
机构
[1] Univ Maryland, Dept Cell Biol & Mol Genet, Dept Mol Genet & Cell Biol, College Pk, MD 20742 USA
[2] Northwestern Univ, Dept Biochem Mol Biol & Cell Biol, Evanston, IL 60208 USA
关键词
D O I
10.1074/jbc.274.16.11439
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The binding of antigen to the B cell antigen receptor (BCR) initiates two major cellular events. First, upon cross linking by antigen, the BCR induces signal transduction cascades leading to the transcription of a number of genes associated with B cell activation. Second, the BCR internalizes and delivers antigens to processing compartments, where processed antigenic peptides are loaded onto major histocompatibility complex (MHC) class II molecules for presentation to T helper cells. The BCR consists of membrane Ig (mIg) and Ig alpha/ Ig beta heterodimer (Ig alpha/Ig beta). The Ig alpha/Ig beta, the signal transducing component of the BCR, has been indicated to play a role in antigen processing. In order to understand the function of the Ig alpha/Ig beta in antigen transport, we studied the intracellular trafficking pathway of the Ig alpha/Ig beta. We show that in the absence of antigen binding, the Ig alpha/Ig beta constitutively traffics with mig from the plasma membrane, through the early endosomes, to the MHC class II peptide-loading compartment. Cross-linking the BCR does not alter the trafficking pathway; however, it accelerates the transport of the Ig alpha/Ig beta to the MHC class II peptide-loading compartment. This suggests that the Ig alpha/Ig beta heterodimer is involved in BCR-mediated antigen transport through the entire antigen transport pathway.
引用
收藏
页码:11439 / 11446
页数:8
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