Use of hydride-based separation materials for organic normal phase chromatography

被引：17

作者：

Pesek, Joseph J. ^{[1
]}

Matyksa, Maria T. ^{[1
]}

Sharma, Anushka ^{[2
]}

机构：

[1] San Jose State Univ, Dept Chem, San Jose, CA 95192 USA

[2] Symyx Technol, Santa Clara, CA USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2008年 / 31卷 / 01期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

hydride; normal phase; chromatography; separation materials;

D O I：

10.1080/10826070701665717

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The chromatographic properties of two hydride-based stationary phases (a material with a hydride surface and one with a hydride surface modified by the addition of C-18) are tested with several types of compounds that are typically retained in the organic normal phase (ONP) mode. Both phases display ONP retention, but to differing degrees. Various mobile phases are investigated, as well as some gradient elution profiles. Efficiency, peak shape, and reproducibility for the several solutes in both isocratic and gradient elution, as well as the effects of water in the mobile phase, are assessed.

引用

页码：134 / 147

页数：14

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