Activation of CEA-CAM-1-mediated cell adhesion via CD98:: involvement of PKCδ

被引：8

作者：

Kakugawa, K

Hattori, M

Beauchemin, N

Minato, N ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Biostudies, Dept Immunol & Cell Biol, Kyoto 6068501, Japan

[2] Kyoto Univ, Grad Sch Sci, Dept Biophys, Kyoto 6068502, Japan

[3] McGill Univ, McGill Canc Ctr, Montreal, PQ H3G 1Y6, Canada

来源：

FEBS LETTERS | 2003年 / 552卷 / 2-3期

关键词：

CD98; CEA-CAM-1; cell adhesion; protein kinase C delta; embryonic carcinoma;

D O I：

10.1016/S0014-5793(03)00924-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CD98 is a multifunctional protein involved in amino acid transport and regulation of integrin-mediated cell adhesion. Herein, we demonstrated that CD98 stimulation by anti-CD98 antibodies induced CEA-CAM-1-mediated cell adhesion in BaF3 cells expressing CEA-CAM-1, and suggest that this might be responsible for compact clumping of F9 embryonic carcinoma cells by CD98 stimulation. CEA-CAM-1 was co-immunoprecipitated by anti-CD98 antibody. CD98 stimulation induced the translocation of cytoplasmic protein kinase CS (PKCdelta) to the cell adhesion sites, and rottlerin that inhibited the PKCdelta translocation abolished the cell aggregation without affecting integrin activation. The results suggested that CD98 stimulation could activate CEA-CAM-1-mediated cell adhesion independently of integrins. (C) 2003 Published by Elsevier B.V. All rights reserved.

引用

页码：184 / 188

页数：5

共 25 条

[11] β1 integrins show specific association with CD98 protein in low density membranes [J].