Altered expression of occludin and tight junction formation in psoriasis

被引:68
作者
Yoshida, Y [1 ]
Morita, K
Mizoguchi, A
Ide, C
Miyachi, Y
机构
[1] Kyoto Univ, Grad Sch Med, Dept Dermatol, Sakyo Ku, Kyoto 6068507, Japan
[2] Kyoto Univ, Grad Sch Med, Dept Anat & Neurobiol, Sakyo Ku, Kyoto 6068501, Japan
关键词
maculae occludentes; psoriasis; occludin; tight junctions;
D O I
10.1007/s004030100221
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
In simple epithelia, tight junctions are well developed and have barrier and fence functions. On the other hand, tight junctions are Less developed in stratified epithelia, In the rodent epidermis, only maculae occludentes (i,e. focal strands or spot tight junctions) are observed in the most superficial zone of the granular cell layer. Occludin is an integral membrane protein, and is localized at tight junctions in simple epithelia, In normal epidermis, occludin is expressed at the maculae occludentes in the granular cell layer, indicating that it is associated with keratinocyte differentiation, Thus, we examined occludin expression in psoriasis, in which differentiation of keratinocytes is impaired. In psoriasis, occludin was expressed more broadly in the upper epidermis than in normal epidermis. In addition, immunoelectron microscopy showed occludin to be concentrated on the maculae occludentes in the spinous layer of psoriatic skin. These findings indicate that occludin and the formation of tight junctions are related to the proliferation and differentiation of keratinocytes, and to the pathogenesis of psoriasis.
引用
收藏
页码:239 / 244
页数:6
相关论文
共 20 条
[1]   Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues [J].
AndoAkatsuka, Y ;
Saitou, M ;
Hirase, T ;
Kishi, M ;
Sakakibara, A ;
Itoh, M ;
Yonemura, S ;
Furuse, M ;
Tsukita, S .
JOURNAL OF CELL BIOLOGY, 1996, 133 (01) :43-47
[2]   ABNORMAL SEQUENCE OF EXPRESSION OF DIFFERENTIATION MARKERS IN PSORIATIC EPIDERMIS - INVERSION OF 2 STEPS IN THE DIFFERENTIATION PROGRAM [J].
BERNARD, BA ;
ASSELINEAU, D ;
SCHAFFARDESHAYES, L ;
DARMON, MY .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1988, 90 (06) :801-805
[3]   SUPRABASAL INTEGRIN EXPRESSION IN THE EPIDERMIS OF TRANSGENIC MICE RESULTS IN DEVELOPMENTAL DEFECTS AND A PHENOTYPE RESEMBLING PSORIASIS [J].
CARROLL, JM ;
ROMERO, MR ;
WATT, FM .
CELL, 1995, 83 (06) :957-968
[4]   PERMEABILITY BARRIER IN MAMMALIAN EPIDERMIS [J].
ELIAS, PM ;
FRIEND, DS .
JOURNAL OF CELL BIOLOGY, 1975, 65 (01) :180-191
[5]   MEMBRANE ALTERATIONS DURING CORNIFICATION OF MAMMALIAN SQUAMOUS EPITHELIA - FREEZE-FRACTURE, TRACER, AND THIN-SECTION STUDY [J].
ELIAS, PM ;
MCNUTT, NS ;
FRIEND, DS .
ANATOMICAL RECORD, 1977, 189 (04) :577-593
[6]   JUNCTIONAL COMPLEXES IN VARIOUS EPITHELIA [J].
FARQUHAR, MG ;
PALADE, GE .
JOURNAL OF CELL BIOLOGY, 1963, 17 (02) :375-&
[7]   A single gene product, claudin-1 or -2, reconstitutes tight junction strands and recruits occludin in fibroblasts [J].
Furuse, M ;
Sasaki, H ;
Fujimoto, K ;
Tsukita, S .
JOURNAL OF CELL BIOLOGY, 1998, 143 (02) :391-401
[8]   OCCLUDIN - A NOVEL INTEGRAL MEMBRANE-PROTEIN LOCALIZING AT TIGHT JUNCTIONS [J].
FURUSE, M ;
HIRASE, T ;
ITOH, M ;
NAGAFUCHI, A ;
YONEMURA, S ;
TSUKITA, S ;
TSUKITA, S .
JOURNAL OF CELL BIOLOGY, 1993, 123 (06) :1777-1788
[9]   Claudin-1 and -2: Novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin [J].
Furuse, M ;
Fujita, K ;
Hiiragi, T ;
Fujimoto, K ;
Tsukita, S .
JOURNAL OF CELL BIOLOGY, 1998, 141 (07) :1539-1550
[10]   BREAKING THROUGH THE TIGHT JUNCTION BARRIER [J].
GUMBINER, BM .
JOURNAL OF CELL BIOLOGY, 1993, 123 (06) :1631-1633