Immunohistochemical detection of dityrosine in lipofuscin pigments in the aged human brain

被引：80

作者：

Kato, Y

Maruyama, W

Naoi, M

Hashizume, Y

Osawa, T

机构：

[1] Nagoya Univ, Dept Appl Biol Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan

[2] Himeji Inst Technol, Sch Humanity Environm Policy & Technol, Himeji, Hyogo 6700092, Japan

[3] Natl Inst Longev Sci, Dept Basic Gerontol, Aichi 4740031, Japan

[4] Inst Appl Biochem, Inst Brain Sci, Gifu 5050116, Japan

[5] Aichi Med Univ, Inst Med Sci Aging, Nagakute, Aichi 4801195, Japan

来源：

FEBS LETTERS | 1998年 / 439卷 / 03期

关键词：

dityrosine; lipofuscin; aging; oxidation;

D O I：

10.1016/S0014-5793(98)01372-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipofuscin is a yellowish brown fluorescent pigment which is sequestered within cytoplasmic granules during aging. To examine the contribution of protein oxidation to lipofuscin accumulation, we performed immunohistochemical detection of dityrosine, which is considered one of the specific markers for protein oxidation, in lipofuscin in the aged human brain using an antibody specific to dityrosine. By characterization using competitive enzyme-linked immunosorbent assay, the specificity of the antibody to dityrosine was confirmed, None of the other tyrosine-related compounds such as L-tyrosine, 3-nitrotyrosine, 3-chlorotyrosine, or 3,4-dihydroxyphenylalanine cross-reacted with the antibody. The anti-dityrosine antibody reacted with lipofuscin granules in the pyramidal neurons of the aged human brain, The results suggest that protein oxidation by free radicals and/or peroxidases may play an important role in lipofuscin accumulation. (C) 1998 Federation of European Biochemical Societies.

引用

页码：231 / 234

页数：4

共 31 条

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