Strand breaks in plasmid DNA following positional changes of Auger-electron-emitting radionuclides

被引：26

作者：

Adelstein, SJ ^{[1
]}

Kassis, AI ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,DEPT RADIOL,BOSTON,MA 02115

来源：

ACTA ONCOLOGICA | 1996年 / 35卷 / 07期

关键词：

D O I：

10.3109/02841869609104029

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The purpose of our studies is to elucidate the kinetics of DNA strand breaks caused by low-energy Auger electron emitters inclose proximity to DNA. Previously we have studied the DNA break yields in plasmids after the decay of indium-lll bound to DNA or free in solution. In this work, we compare the DNA break yields in supercoiled DNA of iodine-125 decaying close to DNA following DNA intercalation, minor-groove binding, or surface binding, and at a distance from DNA. Supercoiled DNA, stored at 4 degrees C to accumulate radiation dose from the decay of I-125, was then resolved by gel electrophoresis into supercoiled, nicked circular, and linear forms, representing undamaged DNA, single-strand breaks, and double-strand breaks respectively. DNA-intercalated or groove-bound I-125 is more effective than surface-bound radionuclide or I-125 free in solution. The hydroxyl radical scavenger DMSO protects against damage by I-125 free in solution but has minimal effect on damage by groove-bound I-125.

引用

页码：797 / 801

页数：5

共 18 条

[1] MOLECULAR RECOGNITION BETWEEN LIGANDS AND NUCLEIC-ACIDS - NOVEL PYRIDINE-CONTAINING AND BENZOXAZOLE-CONTAINING AGENTS RELATED TO HOECHST-33258 THAT EXHIBIT ALTERED DNA-SEQUENCE SPECIFICITY DEDUCED FROM FOOTPRINTING ANALYSIS AND SPECTROSCOPIC STUDIES [J].