Release Profiles of Tricalcium Phosphate Nanoparticles from Poly(L-lactic acid) Electrospun Scaffolds with Single Component, Core-Sheath, or Porous Fiber Morphologies: Effects on hASC Viability and Osteogenic Differentiation

被引：32

作者：

Asli, Mahsa Mohiti ^{[2
]}

Pourdeyhimi, Behnam ^{[2
]}

Loboa, Elizabeth G. ^{[1
,3
]}

机构：

[1] Univ N Carolina Chapel Hill, Joint Dept Biomed Engn, Raleigh, NC 27695 USA

[2] N Carolina State Univ, Coll Text, Raleigh, NC 27695 USA

[3] N Carolina State Univ, Dept Mat Sci & Engn, Raleigh, NC 27695 USA

来源：

MACROMOLECULAR BIOSCIENCE | 2012年 / 12卷 / 07期

关键词：

biomaterials; controlled release scaffolds; human adipose derived stem cells; nanoparticles; osteogenesis; DRUG-DELIVERY; BENDING INSTABILITY; TISSUE; NANOFIBERS; FABRICATION; CALCIUM; DESIGN; CELLS; JETS;

D O I：

10.1002/mabi.201100470

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Functional PLA scaffolds are created with single component, coresheath, or porous fiber morphology and doped with TCP nanoparticles to study the release profiles for use in bone tissue engineering applications. Pharmacokinetic analyses are performed for the three different nanofibrous structures after doping with TCP. Results indicate that single component and porous fiber scaffolds exhibit an initial-burst release profile whereas coresheath fibers show a steady release. All scaffolds are then seeded with human adipose-derived stem cells (hASC), which remain viable and continue proliferation on all nanofibrous morphologies for up to 21 d. Osteogenic differentiation of hASC and cell-mediated calcium accretion are largest on porous fibers.

引用

页码：893 / 900

页数：8

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