Device for sterile online measurement of the oxygen transfer rate in shaking flasks

被引：268

作者：

Anderlei, T ^{[1
]}

Büchs, J ^{[1
]}

机构：

[1] Rhein Westfal TH Aachen, Dept Biochem Engn, D-52056 Aachen, Germany

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2001年 / 7卷 / 02期

关键词：

shaking bioreactor; oxygen transfer; bioprocess monitoring; respiration; bioreactor; fermentation;

D O I：

10.1016/S1369-703X(00)00116-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The oxygen transfer rate (OTR) is the most suitable measurable parameter to quantify the physiological state of a culture of aerobic microorganisms since most metabolic activities depend on oxygen consumption. Online measurement of the oxygen transfer rate in stirred bioreactors is state of the art although technically difficult. However, the online determination of the oxygen transfer rate in shaking bioreactors under sterile conditions has not been possible until recently. A newly developed measuring device eliminates this deficit. Extremely useful information about cultivating conditions and the physiological state of microorganisms can be gained in early stages of research and bioprocess development from many reactors operated in parallel. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：157 / 162

页数：6