Mass transfer resistance of sterile plugs in shaking bioreactors

被引:53
作者
Mrotzek, C
Anderlei, T
Henzler, HJ
Büchs, J
机构
[1] Aachen Tech Univ, Dept Biochem Engn, D-52056 Aachen, Germany
[2] Bayer AG, Bioproc Engn, D-5090 Leverkusen, Germany
关键词
sterile plug; shaking bioreactor; oxygen transfer; fermentation; diffusion; modelling;
D O I
10.1016/S1369-703X(00)00108-X
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
One of the mass transfer resistances for the gas exchange of shaking flasks is the sterile plug. The gas exchange through the sterile plug is described by an extended model of Henzler and Schedel [Bioprocess Eng. 7 (1991) 123]. Based on this model, a new method was developed to obtain the mass transfer resistance of various sterile closures. It consists of measuring the water evaporation rate of the shaking flask and is therefore very easily applied. Sterile plugs made of cotton. wrapped paper, urethane foam and fibreglass and caps made out of aluminium and silicone have been examined. Instead of the oxygen transfer coefficient (k(O2)), which is commonly found in the literature, the carbon dioxide diffusion coefficient (D-CO2) is used to describe the mass transfer resistance of the sterile plug. The investigation revealed that this resistance is mainly dependent on the neck geometry and to a lesser extent on the plug material and density. The gas exchange of aluminium-caps was not reproducible. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:107 / 112
页数:6
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