Assessment of disorder predictions in CASP7

被引:77
作者
Bordoli, Lorenza [1 ]
Kiefer, Florian [1 ]
Schwede, Torsten [1 ]
机构
[1] Univ Basel, Biozentrum, Swiss Inst Bioinformat, CH-4056 Basel, Switzerland
关键词
CASP7; assessment of disorder prediction; intrinsic protein disorder; natively unstructured proteins; ROC;
D O I
10.1002/prot.21671
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Intrinsically unstructured regions in proteins have been associated with numerous important biological cellular functions. As measuring native disorder experimentally is technically challenging, computational methods for prediction of disordered regions in a protein have gained much interest in recent years. As part of the seventh Critical Assessment of Techniques for Protein Structure Prediction (CASP7), we have assessed 19 methods for disorder prediction based on their results for 96 target proteins. Prediction accuracy was assessed using detailed numerical comparison between the predicted disorder and the experimental structures. On average, methods participating in CASP7 have improved accuracy in comparison to the previous assessment in CASP6. Overall, however, no improvement over the best methods in CASP6 was observed in CASP7. Significant differences between different prediction methods were identified with regard to their sensitivity and specificity in correctly predicting ordered and disordered residues based on a protein target sequence, which is of relevance for practical applications of these computational tools.
引用
收藏
页码:129 / 136
页数:8
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