Development and application of a quantitative lateral flow immunoassay for fumonisins in maize

被引：72

作者：

Anfossi, Laura ^{[1
]}

Calderara, Marianna ^{[1
]}

Baggiani, Claudio ^{[1
]}

Giovannoli, Cristina ^{[1
]}

Arletti, Enrico ^{[2
]}

Giraudi, Gianfranco ^{[1
]}

机构：

[1] Univ Turin, Dept Analyt Chem, I-10125 Turin, Italy

[2] Generon Srl S Maria di Mugnano, Modena, Italy

来源：

ANALYTICA CHIMICA ACTA | 2010年 / 682卷 / 1-2期

关键词：

Fumonisins; Lateral flow immunoassay; Quantitative assay; Maize; B-1; FOOD; ELISA; ASSAY; CORN;

D O I：

10.1016/j.aca.2010.09.045

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A quantitative lateral flow Immunoassay for measuring fumonisins in maize was developed Strip preparation and assay parameters were optimized to obtain a dipstick usable outside the laboratory with different samples and which shows performance comparable with that of other screening methods as confirmed by the intra- and the inter-day precision of data (RSD 5-16%) Quantification was obtained by an external calibration curve which can be stored and used for measurements made with strips of the same batch in different days and at varying temperatures (22-37 C) Limit of detection (120 mu g L-1) and dynamic range (200-5000 mu g L-1) allow the direct assessment of fumonisin contamination at all levels of regulatory relevance Twenty-seven maize samples were analyzed after a simple sample preparation which avoids the use of organic solvent Linear correlation was observed (y = 1 071x - 0 2 r(2) = 0 990) when data was compared with that obtained through a reference LC-MS/MS method across a wide range of fumonisin contamination (C) 2010 Elsevier B V All rights reserved

引用

页码：104 / 109

页数：6

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