Subcellular localization of the N-3 methyltransferase involved in caffeine biosynthesis in tea

被引：22

作者：

Kato, A

Crozier, A

Ashihara, H

机构：

[1] Ochanomizu Univ, Grad Sch Life Sci, Dept Mol Biol & Biochem, Bunkyo Ku, Tokyo 112, Japan

[2] Univ Glasgow, Div Biochem & Mol Biol, Plant Mol Sci Grp, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland

来源：

PHYTOCHEMISTRY | 1998年 / 48卷 / 05期

关键词：

Camellia sinensis; Theaceae; tea; SAM; 3-N-methyltransferase; caffeine biosynthesis;

D O I：

10.1016/S0031-9422(97)00975-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Caffeine biosynthesis consists of sequential methylations at N-7, N-3 and N-1 of the xanthine ring catalysed by S-adenosyl-L-methionine-dependent methyltransferase activities. In preparations from young leaves of Camellia sinensis, N-3-methyltransferase was associated with a purified chloroplast preparation obtained by using differential centrifugation and a discontinuous Percoll density gradient. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：777 / 779

页数：3

共 18 条

[1] Biosynthesis of caffeine in leaves of coffee [J].