Inducible expression of Enterococcus faecalis aggregation substance surface protein facilitates bacterial internalization by cultured enterocytes

被引:59
作者
Wells, CL
Moore, EA
Hoag, JA
Hirt, H
Dunny, GM
Erlandsen, SL
机构
[1] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Dept Surg, Minneapolis, MN 55455 USA
[3] Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA
[4] Univ Minnesota, Dept Genet, Minneapolis, MN 55455 USA
[5] Univ Minnesota, Dept Dev & Cell Biol, Minneapolis, MN 55455 USA
关键词
D O I
10.1128/IAI.68.12.7190-7194.2000
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Aggregation substance (AS) is an Enterococcus faecalis surface protein that may contribute to virulence. Using a recently described system for controlled expression of AS in E. faecalis and the heterologous host Lactococcus lactis, experiments were designed to assess the effect of AS on bacterial internalization by HT-29 and Caco-2 enterocytes. AS expression was associated with increased internalization of E. faecalis by HT-29 enterocytes and of L. lactis by HT-29 and Caco-2 enterocytes. Compared to enterocytes cultivated under standard conditions, either cultivation in hypoxia or 1-h pretreatment of enterocytes with calcium-free medium resulted in increased internalization of both E. faecalis and L. lactis (with and without AS expression). Also, AS expression augmented these increases when E. faecalis was incubated with pretreated HT-29 enterocytes and when L. lactis was incubated with pretreated Caco-2 and HT-29 enterocytes. These data indicated that AS might facilitate E. faecalis internalization by cultured enterocytes.
引用
收藏
页码:7190 / 7194
页数:5
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