High-throughput screens and selections of enzyme-encoding genes

被引：124

作者：

Aharoni, A

Griffiths, AD

Tawfik, DS ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel

[2] MRC Ctr, MRC Lab Mol Biol, Cambridge CB2 2QH, England

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2005年 / 9卷 / 02期

基金：

以色列科学基金会;

关键词：

D O I：

10.1016/j.cbpa.2005.02.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The availability of vast gene repertoires from both natural sources (genomic and cDNA libraries) and artificial sources (gene libraries) demands the development and application of novel technologies that enable the screening or selection of large libraries for a variety of enzymatic activities. We describe recent developments in the selection of enzyme-coding genes for directed evolution and functional genomics. We focus on HTS approaches that enable selection from large libraries (> 10(6) gene variants) with relatively humble means (i.e. non-robotic systems), and on in vitro compartmentalization in particular.

引用

页码：210 / 216

页数：7

共 57 条

[1] In vitro display technologies:: novel developments and applications
Amstutz, P
Forrer, P
Zahnd, C
Plückthun, A
[J]. CURRENT OPINION IN BIOTECHNOLOGY, 2001, 12 (04) : 400 - 405
[2] How enzymes adapt: lessons from directed evolution
Arnold, FH
Wintrode, PL
Miyazaki, K
Gershenson, A
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 2001, 26 (02) : 100 - 106
[3] Selection for improved subtiligases by phage display
Atwell, S
Wells, JA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (17) : 9497 - 9502
[4] Chemical complementation: A reaction-independent genetic assay for enzyme catalysis
Baker, K
Bleczinski, C
Lin, HN
Salazar-Jimenez, G
Sengupta, D
Krane, S
Cornish, VW
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (26) : 16537 - 16542
[5] Ultra-high-throughput screening based on cell-surface display and fluorescence-activated cell sorting for the identification of novel biocatalysts
Becker, S
Schmoldt, HU
Adams, TM
Wilhelm, S
Kolmar, H
[J]. CURRENT OPINION IN BIOTECHNOLOGY, 2004, 15 (04) : 323 - 329
[6] In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting
Bernath, K
Hai, MT
Mastrobattista, E
Griffiths, AD
Magdassi, S
Tawfik, DS
[J]. ANALYTICAL BIOCHEMISTRY, 2004, 325 (01) : 151 - 157
[7] BERNATH K, 2005, IN PRESS J MOL BIOL
[8] Methods to increase enantioselectivity of lipases and esterases
Bornscheuer, UT
[J]. CURRENT OPINION IN BIOTECHNOLOGY, 2002, 13 (06) : 543 - 547
[9] Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library
Cesaro-Tadic, S
Lagos, D
Honegger, A
Rickard, JH
Partridge, LJ
Blackburn, GM
Plückthun, A
[J]. NATURE BIOTECHNOLOGY, 2003, 21 (06) : 679 - 685
[10] Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization
Cohen, HM
Tawfik, DS
Griffiths, AD
[J]. PROTEIN ENGINEERING DESIGN & SELECTION, 2004, 17 (01) : 3 - 11

← 1 2 3 4 5 6 →