High-throughput screens and selections of enzyme-encoding genes

被引：124

作者：

Aharoni, A

Griffiths, AD

Tawfik, DS ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel

[2] MRC Ctr, MRC Lab Mol Biol, Cambridge CB2 2QH, England

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2005年 / 9卷 / 02期

基金：

以色列科学基金会;

关键词：

D O I：

10.1016/j.cbpa.2005.02.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The availability of vast gene repertoires from both natural sources (genomic and cDNA libraries) and artificial sources (gene libraries) demands the development and application of novel technologies that enable the screening or selection of large libraries for a variety of enzymatic activities. We describe recent developments in the selection of enzyme-coding genes for directed evolution and functional genomics. We focus on HTS approaches that enable selection from large libraries (> 10(6) gene variants) with relatively humble means (i.e. non-robotic systems), and on in vitro compartmentalization in particular.

引用

页码：210 / 216

页数：7

共 57 条

[21] Recent advances in enzyme assays
Goddard, JP
Reymond, JL
[J]. TRENDS IN BIOTECHNOLOGY, 2004, 22 (07) : 363 - 370
[22] Enzyme assays for high-throughput screening
Goddard, JP
Reymond, JL
[J]. CURRENT OPINION IN BIOTECHNOLOGY, 2004, 15 (04) : 314 - 322
[23] Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization
Griffiths, AD
Tawfik, DS
[J]. EMBO JOURNAL, 2003, 22 (01) : 24 - 35
[24] Man-made enzymes -: from design to in vitro compartmentalisation
Griffiths, AD
Tawfik, DS
[J]. CURRENT OPINION IN BIOTECHNOLOGY, 2000, 11 (04) : 338 - 353
[25] CHANGES IN THE SUBSTRATE SPECIFICITIES OF AN ENZYME DURING DIRECTED EVOLUTION OF NEW FUNCTIONS
HALL, BG
[J]. BIOCHEMISTRY, 1981, 20 (14) : 4042 - 4049
[26] Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries
Harvey, BR
Georgiou, G
Hayhurst, A
Jeong, KJ
Iverson, BL
Rogers, GK
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (25) : 9193 - 9198
[27] High-throughput antibody isolation
Hayhurst, A
Georgiou, G
[J]. CURRENT OPINION IN CHEMICAL BIOLOGY, 2001, 5 (06) : 683 - 689
[28] Jestin JL, 1999, ANGEW CHEM INT EDIT, V38, P1124, DOI 10.1002/(SICI)1521-3773(19990419)38:8<1124::AID-ANIE1124>3.0.CO
[29] 2-W
[30] Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers
Kawarasaki, Y
Griswold, KE
Stevenson, JD
Selzer, T
Benkovic, SJ
Iverson, BL
Georgiou, G
[J]. NUCLEIC ACIDS RESEARCH, 2003, 31 (21) : e126

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