Nonaqueous capillary zone electrophoresis for separation of free fatty acids with indirect fluorescence detection

被引：31

作者：

Wang, TL

Wei, HP

Li, SFY ^{[1
]}

机构：

[1] Natl Univ Singapore, Dept Chem, Singapore 119260, Singapore

[2] Natl Univ Singapore, Dept Chem, Singapore 117548, Singapore

[3] Natl Univ Singapore, Inst Mat Res & Engn, Singapore 117548, Singapore

来源：

ELECTROPHORESIS | 1998年 / 19卷 / 12期

关键词：

capillary zone electrophoresis; nonaqueous electrophoresis; free fatty acids; indirect fluorescence detection;

D O I：

10.1002/elps.1150191224

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The feasibility of combining nonaqueous capillary zone electrophoresis with indirect fluorescence detection was studied for the efficient separation and sensitive detection of ionizable hydrophobic substances which do not possess practically suitable detection properties. Medium- and long-chain free fatty acids, C-6-C-24, were selected as test compounds. The results showed that such a wide range of medium- and long-chain free fatty acids could be separated between any two consecutive homologs in one run and be detected at a level of about 0.01-0.02 mM in highly basic methanol/acetonitrile media containing fluorescein as colon of background electrolyte for indirect fluorescence detection.

引用

页码：2187 / 2192

页数：6

共 36 条

[1] CAPILLARY ELECTROPHORESIS OF ORGANIC AND INORGANIC CATIONS WITH INDIRECT UV DETECTION [J].