Kinetic analysis of the immobilized-lipase-catalyzed synthesis of octanoyl octyl glucoside in acetonitrile

被引:6
作者
Kobayashi, T [1 ]
Adachi, S [1 ]
Matsuno, R [1 ]
机构
[1] Kyoto Univ, Grad Sch Agr, Div Food Sci & Biotechnol, Sakyo Ku, Kyoto 6068502, Japan
关键词
condensation; hydrolysis; kinetics; lipase; organic solvent;
D O I
10.1016/S1369-703X(03)00110-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The condensation of octyl beta-D-glucoside and octanoic acid and the hydrolysis of 6-O-octanoyl octyl beta-D-glucoside using an immobilized lipase from Candida antarctica were carried out in acetonitrile under various conditions. The kinetics for these reactions could be expressed based on the ping-pong bi-bi mechanism, and all the kinetic parameters in the expression were evaluated. The activity of the lipase decreased at water concentrations lower than 0.05 mol/l. The dependence of the activity on the water concentration was expressed by an empirical equation. These equations were useful for predicting the transient changes in the concentrations of the substrates and the products under any conditions even when molecular sieves were added to the reaction system. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:323 / 328
页数:6
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