Stability-indicating liquid chromatographic determination of trifluoperazine hydrochloride in bulk form and tablets

A selective high-performance liquid chromatographic procedure for the stability-indicating determination of trifluoperazine hydrochloride in the presence of its photolytic degradation products is demonstrated. The liquid chromatographic separation was achieved isocratically on a MicroPak(TM) NH2-10 column (10 mu m, 30 cm x 4 mm phi) utilizing a mobile phase containing acetonitrile, tetrabutylammonium hydroxide (TBAH) and o-phosphoric acid (50.5:0.83:0.1, made up to 100 mi with water, v/w/w, pH 6.5) at a rate of 1 ml min(-1) with UV-detection (254 nm) at ambient temperature. Complete resolution of the unchanged trifluoperazine from its photodegradates could be achieved. Satisfactory results were obtained for drug assay and recovery testing.