Mesorhizobium chacoense sp nov., a novel species that nodulates Prosopis alba in the Chaco Arido region (Argentina)

被引:71
作者
Velázquez, E
Igual, JM
Willems, A
Willems, A
Fernádez, MP
Muñoz, E
Mateos, PF
Abril, A
Toro, N
Normand, P
Cervantes, E
Gillis, M
Martínez-Molina, E
机构
[1] Univ Salamanca, Dept Microbiol & Genet, E-37008 Salamanca, Spain
[2] CSIC, IRNA, Salamanca, Spain
[3] Univ Cordoba, Catedra Microbiol Agr, Fac Agron, Cordoba, Argentina
[4] Univ Lyon 1, Lab Ecol Microbienne, URA 697, CNRS, F-69622 Villeurbanne, France
[5] Microbiol Lab, Vakgrp Biochem Fysiol & Microbiol, B-9000 Ghent, Belgium
[6] CSIC, Estac Expt Zaidin, Dept Sistemas, Granada, Spain
关键词
taxonomy; Mesorhizobium; LMW RNA; 16S rRNA; Prosopis;
D O I
10.1099/00207713-51-3-1011
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Low-molecular-weight RNA analysis was performed for the identification and classification of 20 Argentinian strains isolated from the root nodules of Prosopis alba, SDS-PAGE of total cellular proteins, determination of the DNA base composition, DNA-DNA reassociation experiments and physiological and biochemical tests were also carried out for these strains and the whole 16S rRNA gene was sequenced from one of the strains, strain LMG 19008(T). Results of the genotypic and phenotypic characterization showed that the strains isolated in this study belong to a group that clustered in the genus Mesorhizobium. The results of DNA-DNA hybridizations showed that this group is a novel species of this genus. The name Mesorhizobium chacoense sp, nov. is proposed for this species. The type strain is LMG 19008(T) (= CECT 5336(T)).
引用
收藏
页码:1011 / 1021
页数:11
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