A catalytic subunit of the sugar beet protein kinase CK2 is induced by salt stress and increases NaCl tolerance in Saccharomyces cerevisiae

被引：35

作者：

Kanhonou, R

Serrano, R

Palau, RR

机构：

[1] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantes, Valencia 46022, Spain

[2] Univ Valencia, Fac Ciencias Biol, Dept Biol Vegetal, E-46100 Burjassot, Spain

来源：

PLANT MOLECULAR BIOLOGY | 2001年 / 47卷 / 05期

关键词：

Beta vulgaris; CK2 catalytic subunit; NaCl stress; Saccharomyces cerevisiae;

D O I：

10.1023/A:1012227913356

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Salinity is an important limiting factor in plant growth and development. We have cloned a catalytic subunit of the sugar beet protein kinase CK2 (BvCKA2) by functional expression in yeast of a NaCl-induced cDNA library. BvCKA2 was able to increase the yeast tolerance to NaCl and to functionally complement the cka1 cka2 yeast double mutant upon over-expression. Southern blot analysis indicated that, in sugar beet, the BCKA2 gene is a member of a multigene family. The mRNA levels of BvCKA2 were up-regulated in response to NaCl stress which suggests that protein kinase CK2 may be involved in the plant response to salt stress.

引用

页码：571 / 579

页数：9

共 56 条

[1] PROTEIN KINASES .4. PROTEIN-KINASE CK2 - AN ENZYME WITH MULTIPLE SUBSTRATES AND A PUZZLING REGULATION [J].