The Complex I from Rhodobacter capsulatus

被引:86
作者
Dupuis, A [1 ]
Chevallet, M [1 ]
Darrouzet, E [1 ]
Duborjal, H [1 ]
Lunardi, J [1 ]
Issartel, JP [1 ]
机构
[1] CEA Grenoble, Dept Biol Mol & Struct, EA 2019 UJF, Lab Bioenerget Cellulaire & Pathol, F-38054 Grenoble 9, France
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1998年 / 1364卷 / 02期
关键词
Complex I; iron-sulfur cluster; NADH-ubiquinone oxidoreductase; nuo operon; quinone binding site; piericidin; rotenone;
D O I
10.1016/S0005-2728(98)00025-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The NADH-ubiquinone oxidoreductase (type I NDH) of Rhodobacter capsulatus is a multisubunit enzyme encoded by the 14 genes of the nuo operon. This bacterial enzyme constitutes a valuable model for the characterization of the mitochondrial Complex I structure and enzymatic mechanism for the following reasons. (i) The mitochondria-encoded ND subunits are not readily accessible to genetic manipulation. In contrast, the equivalents of the mitochondrial ND1, ND2, ND4, ND4L, ND5 and ND6 genes can be easily mutated in R. capsulatus by homologous recombination. (ii)As illustrated in the case of ND1 gene, point mutations associated with human cytopathies can be reproduced and studied in this model system. (iii) The R. capsulatus model also allows the recombinant manipulations of iron-sulfur (Fe-S) subunits and the assignment of Fe-S clusters as illustrated in the case of the NUOI subunit (the equivalent of the mitochondrial TYKY subunit). (iv) Finally, like mitochondrial Complex I, the NADH-ubiquinone oxidoreductase of R. capsulatus is highly sensitive to the inhibitor piericidin-A which is considered to bind to or close to the quinone binding site(s) of Complex I. Therefore, isolation of R. capsulatus mutants resistant to piericidin-A represents a straightforward way to map the inhibitor binding sites and to try and define the location of quinone binding site(s) in the enzyme. These illustrations that describe the interest in the R. capsulatus NADH-ubiquinone oxidoreductase model for the general study of Complex I will be critically developed in the present review. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:147 / 165
页数:19
相关论文
共 79 条
  • [21] CLONING AND TRANSCRIPTION ANALYSIS OF THE NDH(A-I-G-E) GENE-CLUSTER AND THE NDHD GENE OF THE CYANOBACTERIUM SYNECHOCYSTIS SP PCC6803
    ELLERSIEK, U
    STEINMULLER, K
    [J]. PLANT MOLECULAR BIOLOGY, 1992, 20 (06) : 1097 - 1110
  • [22] NATURAL SUBSTANCES (ACETOGENINS) FROM THE FAMILY ANNONACEAE ARE POWERFUL INHIBITORS OF MITOCHONDRIAL NADH DEHYDROGENASE (COMPLEX-I)
    ESPOSTI, MD
    GHELLI, A
    RATTA, M
    CORTES, D
    ESTORNELL, E
    [J]. BIOCHEMICAL JOURNAL, 1994, 301 : 161 - 167
  • [23] THE MECHANISM OF PROTON AND ELECTRON-TRANSPORT IN MITOCHONDRIAL COMPLEX-I
    ESPOSTI, MD
    GHELLI, A
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1994, 1187 (02): : 116 - 120
  • [24] TRANSCRIPTION OF RHODOSPIRILLUM-RUBRUM-ATP OPERON
    FALK, G
    WALKER, JE
    [J]. BIOCHEMICAL JOURNAL, 1985, 229 (03) : 663 - 668
  • [25] CONSERVATION OF SEQUENCES OF SUBUNITS OF MITOCHONDRIAL COMPLEX-I AND THEIR RELATIONSHIPS WITH OTHER PROTEINS
    FEARNLEY, IM
    WALKER, JE
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1140 (02) : 105 - 134
  • [26] DISRUPTION OF THE GENE ENCODING THE NADH-BINDING SUBUNIT OF NADH-UBIQUINONE OXIDOREDUCTASE IN NEUROSPORA-CRASSA FORMATION OF A PARTIALLY ASSEMBLED ENZYME WITHOUT FMN AND THE IRON-SULFUR CLUSTER N-3
    FECKE, W
    SLED, VD
    OHNISHI, T
    WEISS, H
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 220 (02): : 551 - 558
  • [27] Genetic inactivation of the H+-translocating NADH:ubiquinone oxidoreductase of Paracoccus denitrificans is facilitated by insertion of the ndh gene from Escherichia coli
    Finel, M
    [J]. FEBS LETTERS, 1996, 393 (01) : 81 - 85
  • [28] REFINEMENT OF THE HIGH-RESOLUTION PHYSICAL AND GENETIC-MAP OF RHODOBACTER-CAPSULATUS AND GENOME SURVEYS USING BLOTS OF THE COSMID ENCYCLOPEDIA
    FONSTEIN, M
    KOSHY, EG
    NIKOLSKAYA, T
    MOURACHOV, P
    HASELKORN, R
    [J]. EMBO JOURNAL, 1995, 14 (08) : 1827 - 1841
  • [29] FRIEDMAN DI, 1987, ANNU REV GENET, V21, P453
  • [30] A SMALL ISOFORM OF NADH-UBIQUINONE OXIDOREDUCTASE (COMPLEX-I) WITHOUT MITOCHONDRIALLY ENCODED SUBUNITS IS MADE IN CHLORAMPHENICOL-TREATED NEUROSPORA-CRASSA
    FRIEDRICH, T
    HOFHAUS, G
    ISE, W
    NEHLS, U
    SCHMITZ, B
    WEISS, H
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 180 (01): : 173 - 180