Defective retrovirus insertion activates c-Ha-ras protooncogene in an MNU-induced rat mammary carcinoma

被引：11

作者：

Bera, TK ^{[1
]}

Tsukamoto, T ^{[1
]}

Panda, DK ^{[1
]}

Huang, T ^{[1
]}

Guzman, RC ^{[1
]}

Hwang, SI ^{[1
]}

Nandi, S ^{[1
]}

机构：

[1] Univ Calif Berkeley, Canc Res Lab, Berkeley, CA 94720 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 248卷 / 03期

关键词：

defective retrovirus; activation of Ha-ras protooncogene; insertional mutation; mammary carcinogenesis;

D O I：

10.1006/bbrc.1998.9059

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Endogenous retrovirus sequences are present in the genome of a wide variety of animal species. The activation of the proto-oncogenes of the ras family, particularly c-Ha-ras, by either point mutation or overexpression, has been shown to be associated with a vast number of different cancers. Here we report that the insertion of a defective retrovirus in the -1 intron of rat c-Ha-ras is responsible for the activation of the gene by over 10-fold overexpression in an MNU-induced rat mammary cancer. A portion of the 3' end of the retroviral sequence is expressed as a part of the c-Ha-ras transcript in the carcinoma tissue, indicating the direct involvement of this element in the transcription of the c-Ha-ras gene. The c-Ha-ras structural gene transcribed by the promoter of the defective retroviral element can neoplastically transform the NIH 3T3 cell line upon transfection. (C) 1998 Academic Press.

引用

页码：835 / 840

页数：6

共 30 条

[11] SPECIFIC ACTIVATION OF THE CELLULAR HARVEY-RAS ONCOGENE IN DIMETHYLBENZANTHRACENE-INDUCED MOUSE MAMMARY-TUMORS [J].

DANDEKAR, S ;

SUKUMAR, S ;

ZARBL, H ;

YOUNG, LJT ;

CARDIFF, RD .

MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (11) :4104-4108

[12] ONCOGENES AND CANCER [J].

DUESBERG, PH .

SCIENCE, 1995, 267 (5203) :1407-1408

[13] ACTIVATION OF THE C-H-RAS PROTO-ONCOGENE BY RETROVIRUS INSERTION AND CHROMOSOMAL REARRANGEMENT IN A MOLONEY LEUKEMIA VIRUS-INDUCED T-CELL LEUKEMIA [J].

IHLE, JN ;

SMITHWHITE, B ;

SISSON, B ;

PARKER, D ;

BLAIR, DG ;

SCHULTZ, A ;

KOZAK, C ;

LUNSFORD, RD ;

ASKEW, D ;

WEINSTEIN, Y ;

ISFORT, RJ .

JOURNAL OF VIROLOGY, 1989, 63 (07) :2959-2966

[14] CARCINOGEN-TRANSFORMED AND RADIATION-TRANSFORMED C3H-10T1/2 CELLS CONTAIN RNAS HOMOLOGOUS TO THE LONG TERMINAL REPEAT SEQUENCE OF A MURINE LEUKEMIA-VIRUS [J].

KIRSCHMEIER, P ;

GATTONICELLI, S ;

DINA, D ;

WEINSTEIN, IB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (09) :2773-2777

[15]

LARSSON E, 1989, CURR TOP MICROBIOL, V148, P115

[16]

MANGUES R, 1992, ONCOGENE, V7, P2073

[17]

MCCORMICK DL, 1981, CANCER RES, V41, P1690

[18] NUCLEOTIDE-SEQUENCE OF A COMPLETE MOUSE INTRACISTERNAL-A-PARTICLE GENOME - RELATIONSHIP TO KNOWN ASPECTS OF PARTICLE ASSEMBLY AND FUNCTION [J].

MIETZ, JA ;

GROSSMAN, Z ;

LUEDERS, KK ;

KUFF, EL .

JOURNAL OF VIROLOGY, 1987, 61 (10) :3020-3029

[19] DEVELOPMENT OF A HIGHLY EFFICIENT EXPRESSION CDNA CLONING SYSTEM - APPLICATION TO ONCOGENE ISOLATION [J].

MIKI, T ;

FLEMING, TP ;

CRESCENZI, M ;

MOLLOY, CJ ;

BLAM, SB ;

REYNOLDS, SH ;

AARONSON, SA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (12) :5167-5171

[20]

MIYAMOTO S, 1990, CANCER RES, V50, P6010

← 1 2 3 →