A spruce defensin showing strong antifungal activity and increased transcript accumulation after wounding and jasmonate treatments

被引：31

作者：

Pervieux, I

Bourassa, M

Laurans, F

Hamelin, R

Séguin, A

机构：

[1] Nat Resources Canada, Canadian Forest Serv, Laurentian Forestry Ctr, Ste Foy, PQ G1V 4C7, Canada

[2] INRA Orleans, Unite Ameliorat Genet & Physiol Forestieres, F-45166 Olivet, France

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 2004年 / 64卷 / 06期

关键词：

jasmonic acid; methyl jasmonate; Picea; defensin; wounding;

D O I：

10.1016/j.pmpp.2004.09.008

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In this work, a cDNA encoding a defensin from Picea glauca, named PgD1, was isolated. The corresponding PgD1 DNA encodes a putative protein of 83 amino acids with a calculated molecular mass of 8.9 kDa. The mature protein of 50 amino acids is characterized by the presence of specific highly conserved residues common to all plant defensins. PgD1 is developmentally expressed during seed germination and is also up-regulated by wounding and by jasmonic acid treatment, suggesting a role in both the constitutive and induced defense mechanisms. The recombinant protein was found to cause extensive growth inhibition of three fungal pathogens (Cyclindrocladium floridanum, Fusarium oxysporum and Nectria galligena) at 2.5 muM. Crown Copyright (C) 2004 Published by Elsevier Ltd. All rights reserved.

引用

收藏

页码：331 / 341

页数：11

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